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EC number: 266-946-3 | CAS number: 67701-28-4 This substance is identified by SDA Substance Name: C8-C18 and C18 unsaturated trialkyl glyceride and SDA Reporting Number: 01-001-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to ISO 14669:1999 guideline. GLP compliance not reported. No analytical verification of test concentrations.
- Justification for data waiving:
- other:
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14669:1999 "Water quality" Part 5: Biological methods Section 5.24: Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Glycerides, C8-18, C18-unsatd.
- IUPAC Name:
- Glycerides, C8-18, C18-unsatd.
- Reference substance name:
- Glycerides, C8-18 and C18-unsatd.
- EC Number:
- 266-946-3
- EC Name:
- Glycerides, C8-18 and C18-unsatd.
- Cas Number:
- 67701-28-4
- Molecular formula:
- Triglycerides containing a glycerol backbone esterified to fatty acids with a carbon chain length of C8-18 as well as unsaturated C18 fatty acids
- IUPAC Name:
- Tri(C8-18 and C18-unsatd. fatty acyl)glycerol
- Details on test material:
- - Name of test material (as cited in study report): Palm kernel stearin (CAS N° 92129-07-2, EC N° 295-808-5); under the SDA nomenclature, the name of this substance is ‘Glycerides, C8-18, C18-unsatd.’
- Physical state: Cream/yellow solid
- Expiration date of the lot/batch: Apr. 28, 2004
- Storage condition of test material: Stored in the dark at room temperature
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water accommodated fraction (WAF) methodology
- Preparation: WAFs were prepared by adding test material to the test medium (sterilised natural seawater) to give loading rates of 10, 100 and 1,000 mg/L. In order to mix the test material, which was solid at room temperature, the sample was microwaved on full power for approximately 2 minutes until it became liquid. The contents of the WAF vessels were then stirred using a magnetic stirrer, at 300 rpm, for 24 h, in a controlled environment room set at 20 ± 2 °C and then left to settle for a minimum of 2 h (range 2 – 4 h), to allow the undissolved material to separate out. The aqueous phases - the WAFs – were then drawn off via the tap for use in the tests. Control media was sterilised seawater.
Test organisms
- Test organisms (species):
- other: Acartia tonsa
- Details on test organisms:
- TEST ORGANISM
- Source: Guernsey Sea Farms, Vale, Guernsey
- Age at study initiation (mean and range, SD): 13-15 d
- Food type: Mixed marine algae supplied with the organisms
- Amount, frequency: Ad libitum
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- No data
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.7-8.3 (at time 0), 8.1-8.2 (after 48 h)
- Dissolved oxygen:
- 8.6-8.9 mg/L (at time 0), 7.6-8.9 (after 48 h)
- Salinity:
- 32-33 %
- Nominal and measured concentrations:
- Nominal concentration: 10, 100 & 1,000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Glass crystallising dishes, 150 mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): Not renewed
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): Three
- No. of vessels per control (replicates): Three
- No. of vessels per vehicle control (replicates): Three
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, collected off the beach at Kinmel Bay to the west of Rhyl, Flintshire, N. Wales
- Total organic carbon (mean of three replicates at time 0): 28.3, 28.6 and 29.2 mg/L for 10, 100 and 1000 mg/L loading rate, respectively
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark cycle
- Temperature: 20 ± 2 °C - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol; 1 mg/L
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The WAF prepared from palm kernel stearin was found to be not toxic to A. tonsa, with 48 h EL50 values exceeding 1000 mg/L, the highest loading rate tested.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Other: The percentage of A. tonsa that were immobilised when exposed to 1 mg/L 3,5-DCP was 58 % after 48 h. - Reported statistics and error estimates:
- No data
Any other information on results incl. tables
Table 1: Immobilisation of A. tonsa exposed to WAF of palm kernel stearin and 1 mg/L solution of reference compound 3,5-DCP
Test material | Loading rate (mg/L) | Number of A. tonsa exposed | Cumulative Number (N) and Percentage (P) of A. tonsa immobilised after exposure period of: | |||
24 h | 48 h | |||||
N | P | N | P | |||
Control | 35 | 1 | 3 | 4 | 11 | |
Palm kernel stearin | 10 | 33 | 2 | 6 | 3 | 9 |
100 | 36 | 1 | 3 | 5 | 14 | |
1000 | 39 | 5 | 13 | 10 | 26 | |
3,5-DCP | 1 | 33 | 4 | 12 | 19 | 58 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the 48 h EL50 of the test material was determined to be > 1,000 mg/L.
- Executive summary:
A study was conducted to evaluate the acute toxicity of the palm kernel stearin to Acartia tonsa under static conditions. The procedures followed ISO 14669:1999 "Water quality" Part 5: Biological methods Section 5.24: Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea).
Acartia tonsa were exposed to water accommodated fractions prepared from the test material at concentrations ranging from 10 to 1,000 mg/L for 48 h and immobility was determined at 24 and 48 h.
Under the test conditions, the 48 h EL50 of the test material was determined to be > 1,000 mg/L.
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