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EC number: 244-096-4 | CAS number: 20882-04-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016/01/14 - 2016/02/25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to a suitable and standardized test method under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate
- EC Number:
- 244-096-4
- EC Name:
- [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate
- Cas Number:
- 20882-04-6
- Molecular formula:
- C10H14O6
- IUPAC Name:
- 4-{2-[(2-methylprop-2-enoyl)oxy]ethoxy}-4-oxobutanoic acid
- Reference substance name:
- [SPEC][/SPEC][SYN][/SYN]
- IUPAC Name:
- [SPEC][/SPEC][SYN][/SYN]
- Reference substance name:
- [CS]000000111817[/CS]
- IUPAC Name:
- [CS]000000111817[/CS]
- Test material form:
- other: liquid
- Details on test material:
- Company internal substance number SAT 150073, Batch4006/31
Colorless liquid at room temperature
Constituent 1
Constituent 2
Constituent 3
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.
The samples were diluted by a factor of two with acetonitrile.
Additional samples of the control and the dilution solvent were taken at test start and test end without any sample treatment.
The concentrations of the test item [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate were analysed in the duplicate test media samples from all test concentrations, and in the duplicate control samples, from both sampling times (0 and 48 hours).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test Concentrations: 515.4, 234.3, 106.5, 48.4 and 22.0 mg test item/L and a control
Control:
In the control, test water was used without addition of the test item.
Dosage of Test Item:
The test medium of the highest test concentration of nominal 515.4 mg test item/L was prepared by dissolving 462.8 mg test item into 898 mL test water by intense stirring for 10 minutes. Adequate volumes of this test medium were diluted with test water to prepare the test media.
The test media were prepared just before introduction of the Daphnia (= start of the test).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna (Straus), clone 5
Age at Test Start: From 2 to 17 hours old
Sex: Female
Origin: The Daphnia introduced in the test were taken from ibacon's in-house laboratory culture.
Breeding Conditions: The Daphnia were bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental Daphnia was performed in Elendt M4 medium (see 6.5). The test organisms were not first brood progeny. The Daphnia in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Water Hardness: 2.5 mmol/L (= 250 mg/L) as CaCO3
- Test temperature:
- 20 °C at test start; 20 °C at test end
- pH:
- pH-Values:
8.0 to 8.5 at test start; 7.7 to 8.2 at test end - Dissolved oxygen:
- 8.8 to 9.3 mg/L at test start; 7.7 to 8.0 mg/L at test end
- Nominal and measured concentrations:
- Summary of Analytical Results
Sample Description % of RSD
[mg test item/L] nominal1 [%] n
Control (Control blank) n.a. n.a. 2
Dilution Solvent n.a. n.a. 2
Control n.a. n.a. 4
22.0 107 1 4
48.4 111 3 4
106.5 106 2 4
234.3 105 1 4
515.4 108 1 4
1 mean value of all measured samples per treatment group
RSD: relative standard deviation per treatment group
n: number of analysed samples
n.a.: not applicable - Details on test conditions:
- Test Units
Type and Size:
Glass beakers of 100 mL volume containing approximately 60 mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions.
Identification:
Each test unit was uniquely identified with serial number, treatment and replicate number.
Test Environment:
Controlled environment room
Measurement of pH, Dissolved Oxygen and Water Temperature:
The water temperature, pH-values and dissolved oxygen concentrations were determined at test start and test end in each treatment group. - Reference substance (positive control):
- yes
- Remarks:
- For the evaluation of the quality of the Daphnia clone and the consistency of the experimental conditions, the reference item potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 515.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 515.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 515.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic effect of the test item [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate to Daphnia magna was assessed in a static dose-response test. The 48-hour NOEC was determined to be ≥515.4 mg test item/L. The 48-hour LOEC was determined to be > 515.4 mg test item/L and the 48-hour EC50 value was determined to be >515.4 mg test item/L.
- Executive summary:
The toxic effect of the test item [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate to Daphnia magna was assessed in a static dose-response test. The 48-hour NOEC was determined to be ≥515.4 mg test item/L. The 48-hour LOEC was determined to be > 515.4 mg test item/L and the 48-hour EC50 value was determined to be >515.4 mg test item/L.
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