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EC number: 219-102-3 | CAS number: 2359-15-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-09-27 to 1999-11-23
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 17 July, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 31 July, 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N,N'-methylenebis[methacrylamide]
- EC Number:
- 219-102-3
- EC Name:
- N,N'-methylenebis[methacrylamide]
- Cas Number:
- 2359-15-1
- Molecular formula:
- C9H14N2O2
- IUPAC Name:
- N,N'-methylenebis(2-methylacrylamide)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC; HPLC analyses of the test substance concentration were conducted at the beginning and at the end of every 24 h cycle.
- Details on sampling:
- The samples were filtered (<0.45 pM) and stored at 4 °C until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- At least 2 h before the test the test vessels were filled with the medium to allow equilibration of pH, oxygen and temperature. The pH was not adjusted. The nominal test concentration of 100 mg/L was prepared with tap water. A blank with tap water served as a control.
Test organisms
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: Guppy
- Strain: Poecilia reticulata
- Length at study initiation (length definition, mean, range and SD): 1-3 cm
- Method of breeding: Parental and young fish population was held in two 250 L glass aquaria
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Observations of the fish were made after 2, 24, 48, 72 and 96 h of exposure.
Test conditions
- Hardness:
- 2.4 -4.1 °F
- Test temperature:
- 21 ± 1 °C
- pH:
- 8.4 - 8.8
- Dissolved oxygen:
- 7.5 - 8.0 mg/L
- Nominal and measured concentrations:
- preliminary test: 1, 10, 100 mg/L
definitive test: 0 and 100 mg/L nominal/measured - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L aquaria
- Material, size, headspace, fill volume: all-glass
- Aeration: Tap water from Schlieren, aerated
- Renewal rate of test solution (frequency/flow rate): renewal every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water from Schlieren
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 h photoperiod a day, supplied by overhead white fluorescent tubes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality of the fish was recorded at the same time intervals.
Dead fish were removed immediately from the test medium and weight and length recorded.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No effects up to 100 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No adverse effects observed
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No adverse effects observed
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No adverse effects observed
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of the present 96 h toxicity to fish study the NOEC is considered to be > 100 mg/L. No adverse effects were observed up to the limit concentration of 100 mg/L.
- Executive summary:
In a 96 h acute toxicity study according to OECD guideline 203, adopted 17 July 1992, Guppy (Poecilia reticulata) were exposed to N,N'-Methylenebisacrylamide at nominal concentrations of 0 (control) and 100 mg/L under semi-static conditions in tap water. The added test material as determined by HPLC was 100 ± 5 % at all medium renewals. These concentrations remained stable during both renewal periods. Given these results, effect parameters can be based on the nominal test concentrations.
No mortality or other clinical effect was observed at the limit concentration and the control during the 96-hour test period. The 96 h LC50 was >100 mg a.i./L.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with Poecilia reticulata.
Results Synopsis
Test organism size/age (mean wet weight or length): Guppy (Poecilia reticulata) (1 - 3 cm)
Test Type: Static Renewal
LC50: >100 mg a.i./L
Endpoint(s) Effected: mortality
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