N-(butoxymethyl)acrylamide

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish, juvenile growth test

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

See the Analogue Approach Report attached in Section 13 of the IUCLID dossier.

Reason / purpose for cross-reference:

other: Read across to target substance

Qualifier:

no guideline followed

Principles of method if other than guideline:

A 28-day study was conducted to determine the toxicity of acrylamide monomer to carp (Cyprinus carpio).

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

Dechlorinated municipal tap water

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Strain: Cyprinus carpio
- Source: The carp fingerl ings used in the test were selected from an in-house culture.
- Mean weight: 3.04 g +/- 0.73 g
- Type/source of feed: Tetra SM 80 flake food and Glencoe #3 fish pellets
- Amount given: ad-lib basis
- Frequency of feeding: daily

:

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Post exposure observation period:

No

Hardness:

No data

Test temperature:

13°C (*+/-1°).

pH:

No data

Dissolved oxygen:

No data

Nominal and measured concentrations:

Nominal exposure concentrations were 0, 50, 500 and 5000 µg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material: 20 litre all-glass aquaria containing 15.5 litres
- Aeration: Constant aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per vehicle control (replicates): 2
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12-hour daylight
EFFECT PARAMETERS MEASURED: toxicity
VEHICLE CONTROL PERFORMED: yes

Reference substance (positive control):

no

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

5 000 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

and behavior

Details on results:

Acrylamide did not produce any mortality or observed behavior modification at any concentration.

Validity criteria fulfilled:

yes

Conclusions:

No mortalities or behavioural modifications were noted during the course of the study. The No Observed Effect Level was the highest exposure concentration of 5 mg/l.

Description of key information

A 28-day study was conducted to determine the long-term toxicity of the read-across material, acrylamide, to carp (Cyprinus carpio) in a static freshwater test. The No Observed Effect Level was the highest exposure concentration of 5 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5 mg/L

Additional information

The long term toxicity to fish of the read-across substance, acrylamide, was assessed in a juvenile growth test in carp (Cyprinus carpio) in a static freshwater study for 28 days at nominal exposure concentrations of 0, 50, 500 and 5000 µg/L.

Two sets of 4 all-glass aquaria containing 15.5 litres of dechlorinated municipal tap water were immersed in a water bath held at 13 ± 1 °C. Constant aeration was maintained in each aquarium containing test fish. The fish were introduced into one series of four aquaria immediately after the addition of the assigned concentration of the acrylamide monomer solution. Water changes were accomplished by transferring the test fish between the two sets of four aquaria. The test fish were fed Tetra SM 80 flake food upon introduction to the test system and then prior to each transfer. The temperature of the water-bath was recorded daily.

The read-across substance, acrylamide, did not produce any mortality or observed behaviour modification at any concentration during the study. The No Observed Effect Level was the highest exposure concentration of 5000 µg/L.