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EC number: 216-449-2 | CAS number: 1587-20-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Trimethyl citrate
- EC Number:
- 216-449-2
- EC Name:
- Trimethyl citrate
- Cas Number:
- 1587-20-8
- Molecular formula:
- C9H14O7
- IUPAC Name:
- 1,2,3-trimethyl 2-hydroxypropane-1,2,3-tricarboxylate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- 6.3.1 Specification
Activated sludge from a biologic sewage treatment plant was used as inoculum. The cho-sen plant treats mostly domestic sewage.
6.3.2 Source and Pre-Treatment of inoculum
6.3.2.1 Source
The sludge was taken from the activation basin of the sewage treatment plant in D-67480 Edenkoben.
Date of collection: 23. Feb. 2018, batch no: 20180223.
6.3.2.2 Pre-Treatment
The sludge was filtrated, washed with test medium (2x) and re-suspended in test medium. It was then aerated until use. The dry matter was determined to contain 3980 mg of sus-pended solids/L. - Duration of test (contact time):
- ca. 28 d
Initial test substance concentration
- Initial conc.:
- ca. 20 mg/L
- Based on:
- other: organic carbon
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- This study was performed in order to evaluate aerobic elimination and degradation poten-tial of Trimethyl citrate in a test for ready biodegradability, using a test item concentration of nominally 20 mg organic carbon/L (corresponding to 43.3 mg Trimethyl citrate/L).
The test item in a mineral medium was inoculated and incubated under aerobic conditions in the dark. The amount of DOC in the test solution due to the inoculum was kept as low as possible compared with the amount of organic carbon due to the test item. Allowance was made for the endogenous activity of the inoculum by running parallel blanks with inoculum but without test item. A positive control was run in parallel to check the operation of the procedures. Degradation was followed by determining the carbon dioxide produced. Measurements were taken at sufficiently frequent intervals to allow the identification of the beginning and end of biodegradation.
The test lasted for 28 days.
Reference substance
- Reference substance:
- aniline
Results and discussion
- Test performance:
- All validity criteria were met.
Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation reached 0.4 %. Both replicates of the test item showed very good correspondence.
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 49
- Sampling time:
- 4 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 94
- Sampling time:
- 28 d
- Details on results:
- The test item Trimethyl citrate is considered as “not readily biodegradable“.
The degree of biodegradation reached 94 % after 28 days.
The 10-day-window began on day 4 (graphic evaluation), at its end, 49 % degrada-tion were reached, missing the pass level of 60 % given in the OECD guideline.
Degradation surpassed 60 % within 28 days. Therefore, the test item is considered as “ultimately biodegradable”, within 28 days.
Abiotic degradation reached 0.4 %.
BOD5 / COD results
BOD5 / COD
- Key result
- Remarks on result:
- not measured/tested
- Results with reference substance:
- Degradation of the positive control was 67 % after 11 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item Trimethyl citrate is considered as “not readily biodegradable“. Therefore, when applying the 10-day-window, the test item Trimethyl citrate is not readily biodegradable following OECD 301B and EU C.4-C respectively. As degradation sur-passed 60% in the course of the test, Trimethyl citrate is considered as ultimately biode-gradable.
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