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Diss Factsheets
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EC number: 240-178-9 | CAS number: 16039-53-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- genetic toxicity in vitro
- Remarks:
- Type of genotoxicity: genome mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not reported
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 984
Materials and methods
- Principles of method if other than guideline:
- The test material was studied to determine the ability to cause DNA damage and thereby induce a pleiotropic response in the E. coli strain. The pleiotopic response was determined by checking for the induction of λ prophage.
- GLP compliance:
- not specified
- Type of assay:
- other: Induction of λ prophage in E. coli WP2s (λ)
Test material
- Reference substance name:
- Zinc chloride
- EC Number:
- 231-592-0
- EC Name:
- Zinc chloride
- Cas Number:
- 7646-85-7
- IUPAC Name:
- zinc dichloride
- Details on test material:
- - Name of test material (as cited in study report): Zinc chloride
Constituent 1
Method
- Target gene:
- Not reported
Species / strain
- Species / strain / cell type:
- E. coli WP2
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Minimal Broth Davis (Difco) containing 0.2 % glucose and 20 µg/mL tryptophan (MST)
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- not specified
- Metabolic activation system:
- Not reported
- Test concentrations with justification for top dose:
- 3.2 mM
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Minimal Broth Davis (Difco) containing 0.2 % glucose and 20 µg/mL tryptophan (MST)
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- Streptomycin
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Minimal Broth Davis (Difco) containing 0.2 % glucose and 20 µg/mL tryptophan (MST)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: N-Methyl-N'-nitro-N-nitrosoguanidine
- Untreated negative controls:
- yes
- Remarks:
- Streptomycin
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Minimal Broth Davis (Difco) containing 0.2 % glucose and 20 µg/mL tryptophan (MST)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 20 h
NUMBER OF REPLICATIONS: Duplicate
- Evaluation criteria:
- Clear (non-turbid) wells can occur because of toxicity of the test agent, inhibition of growth, or induction of λ prophage and cell lysis. Thus clear wells indicate toxicity and growth inhibition.
- Statistics:
- Not reported
Results and discussion
Test results
- Species / strain:
- E. coli WP2
- Metabolic activation:
- without
- Genotoxicity:
- ambiguous
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Not reported
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Conclusions:
- Under the test conditions, 3.2 mM of the test material caused two-fold increase in induction of λ prophage over control (reported as +/-).
- Executive summary:
A study was conducted to determine the potential of the test material for induction of λ prophage in E coli WP2 (λ).
The test material in MST was inoculated with 75 µL of a mid-exponential culture of WPs(λ) diluted so that each well receives approximately 2 x 107bacteria. The test plate was incubated at 37 °C and scored after 20 h for turbidity. MNNG and 4NQO served as positive controls and streptomycin as negative control.
Under the test conditions, 3.2 mM of the test material caused a two-fold increase in induction of λ prophage over control (reported as +/-).
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