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EC number: 949-117-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Mar - 21 Aug 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Version April 2004
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- (EC) No.440/2008
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of certificate: 19.11.2018 (The Department of Health of the Government of the United Kingdom)
Test material
- Reference substance name:
- Reaction product of castor oil with glycerol
- EC Number:
- 949-117-7
- Molecular formula:
- not applicable, substance is UVCB
- IUPAC Name:
- Reaction product of castor oil with glycerol
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations, taken at start and end of the 48 hour exposure period
- Sampling method: Samples were taken from the control and each test group from the bulk test preparation at 0 hours and from the pooled replicates at 48 hours for quantitative analysis
- Sample storage conditions before analysis: 48-hour test samples were stored frozen prior to analysis. Duplicate sets of samples were taken at 0 and 48 hours and stored frozen for further analysis if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A nominal amount of test item (100 mg) was dissolved in test water and the volume adjusted to 1 liter to give the 100 mg/L test concentration from which a series of dilutions was made to give further test concentrations of 32, 10, 3.2 and 1.0 mg/L. Each prepared concentration was inverted several times to ensure adequate mixing and homogeneity.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation (mean and range, SD): ≤ 24 h
- Method of breeding: Adult daphnids were maintained in 150 mL glass vessels containing 100 mL Elendt M7 medium in a temperature controlled room maintaining the water temperature at 18 to 22 °C, fed daily with a mixture of algal suspension (Pseudokirchneriella supcapitata), gravid adults were isolated the day before initiation of the test
- Source: in-house culture
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20 °C - 21 °C
- pH:
- 7.8 - 8.5
- Dissolved oxygen:
- 7.9 - 9.0 mg O2/L
- Nominal and measured concentrations:
- -Nominal: 1.0, 3.2, 10, 32 and 100 mg/L
- Measured 0 h: 0.863, 3.04, 9.70, 28.8, 101 mg/L
- Measured 48 h: 1.11, 3.39, 10.7, 34.2, 112 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL glass vessels
- Type (delete if not applicable): open
- Volume of solution: 100 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (Elendt M7 medium), pH of 7.9 ± 0.3
- Intervals of water quality measurement: Water temperature was measured at 0, 24, and 48 h, pH and dissolved oxygen was measured at 0 and 48 h
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light / 8 hours dark cycle with 20 minute dawn and dusk transition periods
- Light intensity: 764 - 900 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
-Immobilization or adverse reactions to exposure recorded at 24 and 48 hours after the start of exposure.
RANGE-FINDING STUDY
- Range-finding study: yes
- Test concentrations: 0.10, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilization was observed at the test concentrations of 0.10 and 1.0 mg/L, immobilization was observed at 10 and 100 mg/L, reduced mobility was observed in the control at 10 mg/L test concentration. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate as the reference item was performed twice in a 12 month period
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 41 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 30 - 57 mg/L (95% confidence limits)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Sub lethal effect of exposure was observed in the 1.0, 3.2, 10 and 32 mg/L test concentrations. This response was being covered with debris. The presence of debris is considered to be due to a potential reaction between the test item and media which may be accompanied by the visual presence of precipitated test item/salts in the test vessels themselves. As all preparations had been noted as being clear colorless solutions throughout the duration of the test, the extent of debris was considered to be minimal.
- Results with reference substance (positive control):
- - positive control test was conducted between 25 Sep - 27 Sep 2018 with concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg potassium dichromate/L
- EC50 (24 h) = 1.1 mg/L; EC50 (48 h)= 0.80 mg/L, the results from the positive control with potassium dichromate were within the normal range for this reference item - Reported statistics and error estimates:
- All results were calculated using ToxRat Professional Version 3.2.1. ToxRat® Solutions GMBH 2001-2015
Any other information on results incl. tables
Table 1. Mean Immobilization of daphnids
Concentration [mg/l] |
Immobile daphnids (n=20) |
|||
absolute |
% |
|||
24 h |
48 h |
24 h |
48 h |
|
Control |
0 |
0 |
0 |
0 |
1.0 |
0 |
0 |
0 |
0 |
3.2 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
32 |
1 |
5 |
5 |
25 |
100 |
20 |
20 |
100 |
100 |
Table 2. Cumulative biological observations
Concentration [mg/l] |
Observations (n=20)
|
|
24 h |
48 h |
|
Control |
20 N |
20 N |
1.0 |
20 N |
17 N; 3 C |
3.2 |
20 N |
18 N; 2 C |
10 |
20 N |
17 N; 3 C |
32 |
19 N |
10 N; 10 C |
100 |
AI |
AI |
N = Normal
C = Covered in debris-considered to be present due to a potential reaction between the test item and media
AI = All Daphnia immobilized
Table 3: Validity criteria for OECD 202
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥7.9 mg/L |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 3 in "any other information on results incl tables"
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