Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-623-8 | CAS number: 108-86-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 Feb - 05 Feb 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- other: Circular on Test Methods of New Chemical Substances (Japan), Alga, growth inhibition test
- GLP compliance:
- yes
- Remarks:
- according to the Japanese GLP Standard
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in the refrigerator
- Stability under test conditions: verified by IR
- Solubility and stability of the test substance in the solvent/vehicle: insoluble in water but soluble in alcohol, ether, chloroform, and benzene - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10, 18, 32, 56 and 100 mg/L
- Sampling method: sample was taken at the beginning of the test, after 24, 48 and 72 hours
- Sample storage conditions before analysis: samples were not stored - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was mixed with medium. After sonication, the stock solution was mixed on a stirrer and stored in a closed bottle. The stock solution was diluted with medium and mixed by hand.
- Controls: medium - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: ATCC22662
- Source (laboratory, culture collection): originally obtained from American Type Culture Collection
- Age of inoculum (at test initiation): 5 days
- Method of cultivation: algae were cultured in the Erlenmeyer flasks and shaked
ACCLIMATION
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: not observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20.5 - 20.7 °C
- pH:
- 7.8 - 10.5
- Nominal and measured concentrations:
- See table in "Any other information on results incl. tables" below
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass flask, 500 mL
- Aeration: no
- Initial cells density: 5 x 1000 cells/mL
- Control end cells density: 624 x 1000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the guideline
OTHER TEST CONDITIONS
- Adjustment of pH: not performed
- Photoperiod: continuously
- Light intensity and quality: 60 - 65 μE/m2/s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
Range finding study:
- Test concentrations: 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: After 72 hours, test substance was between 51 and 62% of the nominal concentration with or without algae - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL
- Remarks:
- 12.0 - 12.5 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.87 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Reported statistics and error estimates:
- ANOVA, Williams test, subsequent to Barlett test
Reference
Table 1. Measured concentration of the test substance in test cultures
Nominal concentration (mg/L) |
Measured concentration (mg/L) (Percent of nominal) |
Meanameasured concentration (mg/L) (Percent of nominal) |
|||
0 h |
24 h |
48 h |
72 h |
||
- |
< 0.05 |
< 0.05 |
< 0.05 |
< 0.05 |
- |
10 |
7.55 (76) |
4.50 (45) |
4.53 (45) |
3.89 (39) |
4.87 (49) |
18 |
13.8 (77) |
8.20 (46) |
8.08 (45) |
7.30 (41) |
8.86 (49) |
32 |
24.8 (78) |
14.6 (46) |
14.6 (46) |
12.4 (39) |
15.8 (49) |
56 |
42.9 (77) |
25.6 (46) |
24.7 (44) |
21.1 (38) |
27.2 (49) |
100 |
73.9 (74) |
43.2 (43) |
43.2 (43) |
37.6 (38) |
46.9 (47) |
a: Time weighted mean
Table 2. Biomass of Pseudokirchnerialla subcapitata during the 72 -hour exposure
Nominal concentration [Meanameasured concentration] (mg/L)
|
Biomass (cells/mL) Average ± SD |
|||
0 h* |
24 h |
48 h |
72 h |
|
- |
5000 ± 0 |
2260 ± 1580 |
18100 ± 25500 |
624000 ± 48800 |
10 [4.87] |
5000 ± 0 |
18600 ± 1970 |
152000 ± 6350 |
643000 ± 41600 |
18 [8.86] |
5000 ± 0 |
18200 ± 2020 |
103000 ± 15900 |
392000 ± 55400 |
32 [15.8] |
5000 ± 0 |
12400 ± 500 |
14400 ± 854 |
12400 ± 529 |
56 [27.2] |
5000 ± 0 |
6750 ± 292 |
6790 ± 470 |
5930 ± 341 |
100 [46.9] |
5000 ± 0 |
4960 ± 317 |
5170 ± 117 |
4340 ± 635 |
a: Time weighted mean
SD: Standard deviation
*: Nominal initial biomass
Table 3. Growth inhibition (%) of Pseudokirchneriella subcapitata
Nominal concentration [Meanameasured concentration] (mg/L) |
|
Growth rate |
|
Rate μ(0-72h) |
Inhibition (%)*1 Iμ (0-72h) |
||
- |
Average SD |
0.0670 0.0011 |
- |
10 [4.87] |
Average SD |
0.0674 0.0009 |
-0.6 |
18 [8.86] |
Average SD |
0.0605** 0.0019 |
9.6 |
32 [15.8] |
Average SD |
0.126** 0.0006 |
81.1 |
56 [27.2] |
Average SD |
0.0024** 0.0008 |
96.5 |
100 [46.9] |
Average SD |
-0.0021** 0.0020 |
103.1 |
a: Time weighted mean
*1: Values are the growth inhibition (%) relative to the control
SD: Standard deviation
**: Indicates a significant difference (α=0.01) from the control
Description of key information
ErC50 (72 h): 12.3 mg/L (measured (TWA), Circular on Methods of New Chemical Substances, Japan).
NOEC (72 h, growth): 4.87 mg/L (measured (TWA), Circular on Methods of New Chemical Substances, Japan).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 12.3 mg/L
- EC10 or NOEC for freshwater algae:
- 4.87 mg/L
Additional information
There is one GLP study available investigating the toxicity of the test substance to freshwater green algae according to “Circular on Test Methods of New Chemical Substances (Japan)”.
In a static test Pseudokirchneriellea subcapitata were exposed to nominal test concentrations of 10, 18, 32, 56, and 100 mg/L in closed glass flasks for 72 h under controlled conditions. Cell concentrations were measured by electronic particle counter and test solution concentrations were chemically analysed by HPLC, at the beginning of the test and after 24, 48, and 72 h.
Chemical analysis indicated that the test substance was not stable over time. Effect concentrations were provided as time weighted mean values. The derived ErC50 (72 h) was 12.3 mg/L and the NOEC (72 h, growth rate) was 4.87 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.