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Diss Factsheets
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EC number: 200-664-3 | CAS number: 67-68-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- The toxicity of single and repeated dermal applications of dimethyl sulfoxyde
- Author:
- Smith ER, Hadidian Z and Mason MM
- Year:
- 1 968
- Bibliographic source:
- J Clin Pharmacol, 8(5), 315-321
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Rats were immersed in a DMSO solution (40, 60, 80 or 100%) until the fur and the skin were thoroughly wetted. The animals were then withdrawn from the solution and allow most of the excess solution to run off. From the weights before and after dipping, it was possible to calculate the amount of DMSO.
- GLP compliance:
- no
- Test type:
- other:
Test material
- Reference substance name:
- Dimethyl sulfoxide
- EC Number:
- 200-664-3
- EC Name:
- Dimethyl sulfoxide
- Cas Number:
- 67-68-5
- Molecular formula:
- C2H6OS
- IUPAC Name:
- dimethyl sulfoxide
- Details on test material:
- Test article name: Dimethyl sulfoxide
Source: Schering AG (West Berlin, Germany), The Aldrich Chemical Company (Milwaukee, Wisconsin)
Purity: no data
Other: Identity analysis conducted (densities, freezing & boiling points). Density of 1.1G/mL assumed.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 108-182 g
- Fasting period before study: no data
- Housing: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: From: To: no data
Administration / exposure
- Type of coverage:
- other:
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: whole body except head
- % coverage: entire unshaven body immersed in solutions of DMSO
- Total volume applied: according to the body weight determined before and after dipping in solution : 2, 13, 33, 44 g/kg.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no, animals were just held off after immersion to allow most of the excess solution to run off - Duration of exposure:
- few seconds (time to fur and skin got thoroughly wetted)
- Doses:
- 40, 60, 80 and 100% DMSO (according to the body weight determined before and after dipping in solution the dose levels are 2, 13, 33, 44 g/kg)
- No. of animals per sex per dose:
- 14 (males & females) for the undiluted solution & 4 (males & females) for each other 3 doses
- Control animals:
- no
- Details on study design:
- EXAMINATIONS:
- Duration of observation period following administration: 24H
- Frequency of observations and weighing: clinical signs recorded and body weight measured before and after administration.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, microscopic examination: "complete" - Statistics:
- Not reported : "LD50 estimated to be approximately"
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 40 000 mg/kg bw
- Mortality:
- There was no immediate response, but within 24 hours 13/14 rats dipped into 100% DMSO were dead.
No death occurred at the other concentrations. - Clinical signs:
- other: No data reported. No data on local skin effects.
- Gross pathology:
- Intestines of the animals which did not survive were filled with yellow liquid without food, supposed to be bile and intestinal secretion.
A complete microscopic examination of tissues revealed no changes.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information
- Conclusions:
- Under these experimental conditions, the dermal LD50 of DMSO is ca. 40000 mg/kg in Sprague Dawley rats.
- Executive summary:
The acute dermal toxicity of DMSO was evaluated in male and female rats according to the immersion method. Groups of 4 to 14 unshaven rats (108-182 g) were immersed in a DMSO solution (40, 60, 80 or 100%) until the fur and the skin were thoroughly wetted.
The animals were then withdrawn from the solution and allow most of the excess solution to run off.
According to the body weight determined before and after dipping in solution: 2, 13, 33, 44 g/kg. Animals were then observed for 24 hours.
There was no immediate response, but within 24 hours 13/14 rats dipped into 100% DMSO were dead. No death occurred at the other concentrations. Intestines of the animals that did not survive were filled with yellow liquid without food, supposed to be bile and intestinal secretion.
A complete microscopic examination of tissues revealed no changes.
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