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EC number: 200-824-2 | CAS number: 74-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientific work conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- publication
- Title:
- Inhalation exposure in Drosophila mutagenesis assays: experiments with aliphatic halogenated hydrocarbons, with emphasis on the genetic activity profile of 1,2-dichloroethane.
- Author:
- Kramers, P.G.N., Mout. H.C.A., Bissumbhar. B., Mulder. C.R.
- Year:
- 1 991
- Bibliographic source:
- Mutation Research 252(1), 17-33.
Materials and methods
- Principles of method if other than guideline:
- Flies were kept in flasks containing approximately 8 ml of standard Drosophila melanogaster medium into which the desired amount of test material (non-specified purity, DBM) was injected through a rubber septum. The SLRL tests were carried out with treated adult male Drosophila melanogaster flies of a wild-type strain (Berlin K) that were 1-3 days old at the beginning of treatment. The treated males were mated with 3 virgin females of the Base strain and subsequently re-mated every 2 or 3 days with 3 fresh virgins up to a maximum of 5 such mating periods (broods). After short-term exposure, this mating procedure allows the separate sampling of germ-cell populations treated at different stages of their development.
- GLP compliance:
- no
- Type of assay:
- Drosophila SLRL assay
Test material
- Reference substance name:
- Dibromomethane
- EC Number:
- 200-824-2
- EC Name:
- Dibromomethane
- Cas Number:
- 74-95-3
- Molecular formula:
- CH2Br2
- IUPAC Name:
- dibromomethane
- Details on test material:
- non-specified purity, DBM
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Sex:
- male/female
Administration / exposure
- Vehicle:
- standard Drosophila medium
- Details on exposure:
- Groups of 20-25 flies were kept in 1160 ml flasks containing approximately 8 ml of standard Drosophila medium into which the desired amount of test material (non-specified purity, DBM) was injected through a rubber septum. When treatment times exceeded 48 hours, the vessels were flushed every 2 or 3 days (fresh medium was given in the case of adult treatment) and a new dose of test material was introduced. The SLRL tests were carried out with treated adult male Drosophila melanogaster flies of a wild-type strain (Berlin K) that were 1-3 days old at the beginning of treatment. The treated males were mated with 3 virgin females of the Base strain and subsequently re-mated every 2 or 3 days with 3 fresh virgins up to a maximum of 5 such mating periods (broods). After short-term exposure, this mating procedure allows the separate sampling of germ-cell populations treated at different stages of their development.
Examinations
- Tissues and cell types examined:
- For the detection of SLRL mutations, the standard scheme was performed.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
Any other information on results incl. tables
The results given at the table below demonstrate the number of mortalities per number of chromosomes test in each mating period (brood) and in total.
Frequencies of sex-linked recessive mortalities after treatment with DBM
|
Expose. Time |
Conc. (mg/m3) |
Brood A nl/nchr# %l |
Brood B nl/nchr %l |
Brood C nl/nchr %l |
Brood D nl/nchr %l |
Brood E nl/nchr %l |
Brood A-E nl/nchr %l |
DBM |
6h |
60.2 |
0/396 0 |
1/386 0.26 |
1/396 0.25 |
0/394 0 |
0/392 0 |
2/1964 0.1 |
#Number of lethal/number of chromosomes tested
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
DBM is not mutagenic when evaluated in the Drosophila melanogaster sex-linked recessive lethal test. - Executive summary:
In order to detect the occurrence of mutations, both point mutations and small deletions, in the germ line of an insect, the sex-linked recessive lethal (SLRL) test using Drosophila melanogaster was conducted. The inhalation route was selected by analogy to the human exposure situation.
Groups of 20-25 flies were kept in 1160 ml flasks containing approximately 8 ml of standard Drosophila medium into which the desired amount of test material (non-specified purity, DBM) was injected through a rubber septum. When treatment times exceeded 48 hours, the vessels were flushed every 2 or 3 days (fresh medium was given in the case of adult treatment) and a new dose of test material was introduced. The SLRL tests were carried out with treated adult male Drosophila melanogaster flies of a wild-type strain (Berlin K) that were 1-3 days old at the beginning of treatment. The treated males were mated with 3 virgin females of the Base strain and subsequently re-mated every 2 or 3 days with 3 fresh virgins up to a maximum of 5 such mating periods (broods). After short-term exposure, this mating procedure allows the separate sampling of germ-cell populations treated at different stages of their development. Upon long-term exposure, however, one may expect accumulation of damage according to the specific sensitivities of the stages through which the cells have proceeded during treatment. For the detection of SLRL mutations, the standard scheme was performed.
DBM is not mutagenic when evaluated in the Drosophila melanogaster sex-linked recessive lethal test.
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