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EC number: 247-368-0 | CAS number: 25956-17-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- calculation (if not (Q)SAR)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from computational model developed by USEPA
- Justification for type of information:
- Data is from computational model developed by USEPA
- Qualifier:
- according to guideline
- Guideline:
- other: Modeling database
- Principles of method if other than guideline:
- Estimation of 96 hr EC50 value of test chemical by modelling Database - ECOSAR v.1.11. The ECOSAR Class Program is a computerized version of the ECOSAR analysis procedures as currently practiced by the Office of Pollution Prevention and Toxics (OPPT). It has been developed within the regulatory constraints of the Toxic Substances Control Act (TSCA). It is a pragmatic approach to SAR. The structure-activity relationships (SARs) presented in this program are used to predict the aquatic toxicity of chemicals based on their similarity of structure to chemicals for which the aquatic toxicity has been previously measured. Most SAR calculations in the ECOSAR Class Program are based upon the octanol/water partition coefficient (Kow). Various surfactant SAR calculations are based upon the average length of carbon chains or the number of ethoxylate units.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- other: green algae
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Estimated data
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 149.94 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Neutral Organics SAR (Baseline Toxicity)
- Validity criteria fulfilled:
- no
- Conclusions:
- The estimated EC50 values for aquatic green algae after 96 hours was 149.940 mg/l on the basis of growth rate.
- Executive summary:
The short-term toxicity of the test substance to green algae was predicted using EPI Suite ECOSAR version 1.10. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance was estimated to be 149.940 mg/L. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered as not-classified as per the CLP classification criteria.
Reference
Description of key information
The estimated EC50 values for aquatic green algae after 96 hours was 149.940 mg/l on the basis of growth rate.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 149.94 mg/L
Additional information
Based on the predicted data for the target and experimental data for the read-across analogues, toxicity of test chemical were determined on the basis of growth inhibition of green algae. The studies are summarized as below:
The short-term toxicity of the test substance to green algae was predicted using EPI Suite ECOSAR version 1.10. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance was estimated to be 149.940 mg/L. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered as not-classified as per the CLP classification criteria.
To support the above value, freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance according to OECD Guideline 201. The stock solution 100 mg/l was prepared by dissolving red powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 5x10(3) cells /ml algal culture was use in the study for total exposure period of 72hrs. Test concentrations used were 0, 0, 20, 30, 45, 67, 100 mg/l. Effects on the growth rate of the organism were studied.
Thus, the median effective concentration (ErC50) for the test substance after 72h, in Desmodesmus subspicatus was determined to be 285.8 mg/L. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as toxic as per the CLP criteria.
Again as a supporting study, algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance according to OECD Guideline 201. The stock solution 200 mg/L was prepared by dissolving dark blue powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Test concentrations 12.5, 25, 50, 100 and 200 mg/L were prepared by diluting stock solution with OECD medium. Along with these concentrations control was also run simultaneously. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, on Desmodesmus subspicatus after 72h was determined to be 276.1 mg/L.
Thus, based on the above ErC50 values and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the test substance does not exhibit toxicity to aquatic algae and hence cannot be classified.
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