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EC number: 939-717-7 | CAS number: 1474044-79-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 6, 2012 to May 22, 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Duration of test (contact time):
- >= 28 d
- Initial conc.:
- >= 19.5 mg/L
- Based on:
- ThIC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA)),
- 1 positive control (sodium acetate) and
- 1 toxicity control (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) plus sodium acetate). - Preliminary study:
- No preliminary test was conducted.
- Test performance:
- 1. The positive control substance was biodegraded by at least 60% (73%) within 14 days.
2. The difference of duplicate values for %-degradation of the test substance was always less than 20.
3. The total CO2 release in the blank at the end of the test did not exceed 40 mg/l (46 mg CO2 per 2 litres of medium, corresponding to 23 mg CO2/l).
4. The Inorganic Carbon content (IC) of the test substance (suspension) in the mineral medium at the beginning of the test was less than 5% of the Total Carbon content (TC). Since the test medium was prepared in tap-water purified by reverse osmosis (Milli-RO water (Millipore Corp., Bedford, Mass., USA, carbon levels < 500 ppb)), IC was less than 5% of TC (mainly coming from the test substance, 13 mg TOC/l).
Since all criteria for acceptability of the test were met, this study was considered to be valid. - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 14
- Sampling time:
- 29 d
- Details on results:
- See results table
- Results with reference substance:
- The relative biodegradation values calculated from the measurements performed during the test period revealed 14 and 17% biodegradation of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA), for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
In the toxicity control more than 25% biodegradation occurred within 14 days (33%, based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- Under the conditions of the test, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not readily biodegradable.
- Executive summary:
DNNSA is the main component of CALCIUM BIS( DI C8-C10, BRANCHED, C9 RICH, ALKYLNAPHTHALENE SULPHONATE). Testing with DNNSA can used as read-across for its calcium salt.
A ‘ready’ biodegradability (modified Sturm test) was conducted with di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA). The study procedures described in this report were based on the OECD guideline No. 301 B, 1992. In addition, the procedures were designed to meet the test methods of the Commission Regulation (EC) No. 440/2008 of 30 May 2008, Publication No.L142, Part C.4-C and the ISO International Standard 9439, 1999 and ISO Standard 10634, 1995.
di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was a brown highly viscous liquid with a purity of provisional 84%. The test substance was tested in duplicate at 19.5 mg/l, corresponding to 13 mg TOC/l. The organic carbon content was based on the molecular formula and the purity of the testsubstance. The Theoretical CO2production (ThCO2) of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was calculated to be 2.49 mg CO2/mg.
The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA)),
- 1 positive control (sodium acetate) and
- 1 toxicity control (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) plus sodium acetate).
Since di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms. Test duration was 28 days (last CO2-measurement on the 29thday).
The relative biodegradation values calculated from the measurements performed during the test period revealed 14 and 17% biodegradation of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA), for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met. In the toxicity control, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was found not to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was designated as not readily biodegradable. CALCIUM BIS( DI C8-C10, BRANCHED, C9 RICH, ALKYLNAPHTHALENE SULPHONATE) is also not readily biodegradable.
Reference
Table 1 CO2 production and percentage biodegradation of the test substance (bottle A). |
|
|||||||
|
Day |
HCl (0.05 N) titrated (ml) |
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
|
Blank (mean) |
bottle A |
||||||
|
2 |
47.89 |
47.09 |
0.79 |
0.9 |
0.9 |
1 |
|
|
5 |
47.04 |
45.71 |
1.33 |
1.5 |
2.3 |
3 |
|
|
7 |
47.15 |
45.91 |
1.24 |
1.4 |
3.7 |
4 |
|
|
9 |
46.78 |
45.83 |
0.95 |
1.0 |
4.7 |
5 |
|
|
14 |
46.06 |
45.29 |
0.77 |
0.8 |
5.6 |
6 |
|
|
19 |
44.81 |
43.71 |
1.10 |
1.2 |
6.8 |
8 |
|
|
23 |
44.98 |
43.41 |
1.57 |
1.7 |
8.5 |
10 |
|
|
27 |
45.32 |
44.23 |
0.00 |
0.0 |
8.5 |
10 |
|
|
29 |
45.93 |
44.93 |
1.00 |
1.1 |
9.6 |
11 |
|
|
29 |
48.63 |
47.12 |
1.51 |
1.7 |
11.3 |
13 |
|
|
29 |
49.53 |
48.46 |
1.07 |
1.2 |
12.5 |
14 |
|
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test substance: 87.3 mg CO2/2l |
|||||||
Table 2 CO2 production and percentage biodegradation of the test substance (bottle B). |
|
|||||||
|
Day |
HCl (0.05 N) titrated (ml) |
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
|
Blank (mean) |
bottle B |
||||||
|
2 |
47.89 |
47.49 |
0.39 |
0.4 |
0.4 |
0 |
|
|
5 |
47.04 |
46.21 |
0.82 |
0.9 |
1.3 |
2 |
|
|
7 |
47.15 |
45.75 |
1.40 |
1.5 |
2.9 |
3 |
|
|
9 |
46.78 |
46.10 |
0.68 |
0.7 |
3.6 |
4 |
|
|
14 |
46.06 |
44.78 |
1.28 |
1.4 |
5.0 |
6 |
|
|
19 |
44.81 |
43.15 |
1.66 |
1.8 |
6.9 |
8 |
|
|
23 |
44.98 |
43.35 |
1.63 |
1.8 |
8.6 |
10 |
|
|
27 |
45.32 |
43.11 |
2.21 |
2.4 |
11.1 |
13 |
|
|
29 |
45.93 |
44.60 |
1.33 |
1.5 |
12.5 |
14 |
|
|
29 |
48.63 |
46.86 |
1.77 |
1.9 |
14.5 |
16 |
|
|
29 |
49.53 |
48.76 |
0.77 |
0.8 |
15.3 |
17 |
|
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test substance:88.2 mg CO2/2l |
Description of key information
DNNSA is an intermediate in the production process of CALCIUM BIS( DI C8-C10, BRANCHED, C9 RICH, ALKYLNAPHTHALENE SULPHONATE). Testing with DNNSA demonstrated the substance is not readily biodegradable. CALCIUM BIS( DI C8-C10, BRANCHED, C9 RICH, ALKYLNAPHTHALENE SULPHONATE) is therefore also not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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