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EC number: 259-201-9 | CAS number: 54528-00-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 19 Feb. 2010 to 25 Mar. 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Deviations :
The immobilised daphnia were counted for the first time after 25 hours and 45 minutes instead after 24 hours. This deviation was meaningless for the result of the test. The deviation was signed and assessed by the study director on 30. Mar. 2010.
On request of the sponsor, no analytical determination of the content of the test item in the test solutions during the test was made. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Hexahydrocyclopenta[c]pyrrol-2(1H)-amine
- EC Number:
- 259-201-9
- EC Name:
- Hexahydrocyclopenta[c]pyrrol-2(1H)-amine
- Cas Number:
- 54528-00-6
- Molecular formula:
- C7H14N2
- IUPAC Name:
- octahydrocyclopenta[c]pyrrol-2-amine
- Test material form:
- liquid
- Remarks:
- Colourless
Constituent 1
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Deionised water with an enrichment of certain minerals (as demanded in the guidelines) is used in the test. The dilution water specification is :
CaCl2*2H20 = 293.80 mg/L
MgS04*7H20 = 123.30 mg/L
NaHC03 = 64.80 mg/L
KCl = 5.80 mg/L
Resultin hardness = 2.50 mmol/L
Resultin hardness = 250 mg CaCO3/L
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna
Variety: STRAUS
Strain: Berlin
Sex: female
Age: between 0 and 24 hours
Origin: Umweltbundesamt Berlin
Arrival of Strain: 27. sep. 2007
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Resultin hardness = 2.50 mmol/L
Resultin hardness = 250 mg CaCO3/L - Test temperature:
- 20.8°C at T0 hour
19.9°C at T24hours
19.9 at T48 hours - pH:
- pH is 7.7(mini) to 8.5 (maxi) at T0 hour
pH is 7.7 (mini) to 8.0(maxi) at T48 hours - Dissolved oxygen:
- O2 concentration is 8.8mg/L (mini) to 9.3mg/L (maxi) at T0 hour
O2 concentration is 8.8mg/L (mini) to 8.9mg/L (maxi) at T48 hours - Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal concentrations : 0 ; 1 ; 10 & 100mg/L
- Details on test conditions:
- No feeding and no lighting.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr207 (CAS No. 7778-50-9) was used as positive control.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 - < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 - < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- NOEC 24h = 0.5mg/L
24h EC50i = 1.2mg/L
24h EC100i = 2.5mg/L
Any other information on results incl. tables
Immobilities
In the control, none of the daphnia died or showed any signs of abnormal behaviour throughout the test.
Table of immobilities:
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours |
||||||||
abs. |
|
in % |
abs. |
in % |
||||||
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
3 |
5 |
3 |
4 |
75 |
4 |
5 |
4 |
4 |
85 |
pH and O2, Temperature
The pH values in the test media and the control ranged from 7.7 to 8.5. The concentrationof dissolved oxygen stayed above 8.8 mg/L throughout the test. Temperature range was 19.9 — 20.8oc. The details are given in the following tables:
Table of pH and O2values:
Nominal Concentration in mg/L |
pH |
02-Concentration in m IL |
||
0 h |
48 h |
0 h |
48 h |
|
0 |
7.7 |
7.7 |
9.3 |
8.8 |
1 |
7.7 |
7.7 |
8.8 |
8.9 |
10 |
7.8 |
7.8 |
8.9 |
8.8 |
100 |
8.5 |
8.0 |
9.2 |
8.9 |
Table of temperatures :
Temperature in C |
||
0 h |
24 h |
48 h |
20.8 |
19.9 |
19.9 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All validity criteria were met. For the estimation of the EC50 of the positive control, the fits showed sufficient statistical correspondence of the data with the dose-response-equation. The 24h-EC50i of potassium dichromate was determined as 1.3 mg/L, lying within the demanded range of 0.6 — 1.7 mg/L.
The result of the test can be considered valid. - Executive summary:
The main study was performed using three concentrations containing 1, 10 and 100 mg/L. Twenty daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
The highest concentration showed toxicity: 75 % immobilisation after 24 h and 85% immobilisation after 48 h. No animals were immobilised in the control.
The 24h-EC50i of potassium dichromate was tested in a current reference test. The value was determined as 1.3 mg/L, lying within the demanded range of 0.6 — 1.7 mg/L.
The following results were determined for the test item 3-AMINO 3-AZABICYCLO(3,3,0)OCTANE ("AMINAZA PURIFIEE") (species: Daphnia magna).
24h-NOEC = 10 mg/L
48h-NOEC = 10 mg/L
24h-EC50i: 10 -100 mg/L
48h-EC50i: 10 -100 mg/L
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