(E)-5[(4-chlorophenyl)methylene]-2,2-dimethylcyclopentanone

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

131984-21-9

Cas Number:

131984-21-9

IUPAC Name:

131984-21-9

Details on test material:

- Name of test material (as cited in study report): RPA 405217
- Lot/batch No.: DA 165

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by diluting 0.32 g test substance/l with the help of Tween 80. The test solutions were prepared separately.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.1 g/l

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: No. 86.81 SAG
- Source (laboratory, culture collection): algae culture of the University of Göttingen.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22.5 ± 1 °C

pH:

8.8 - 9.3

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
5, 10, 20, 40, 80 and 160

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Fill volume: 250 ml
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline OECD 201


OTHER TEST CONDITIONS
- Photoperiod: continous illumination
- Light intensity and quality: 8000 lux (0.72 x E20 photons/m²s)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

3.44 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

35.41 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

364.49 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

Self fluorescence of the test substance: 0.01 in the highest test concentration