Fatty acids, C12-18 and C18-unsatd., 2-sulfoethyl esters, sodium salts

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982-11-23 to 1982-12-22

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study conducted according to GLP and OECD guideline (301B)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

temperature range 16.0 to 24.0 degrees C; pH range of test media after addition of test substance stock solutions was 7.44-7.60

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Defi base (sodium acyl ethoxy sulphonate)
Defi is a sodium acyl isethionate also known as coconut acid, 2 sulphoethyl ester, sodium salt. It is a white solid obtained from Lever Brothers, Edgewater. The main constituent is sodium acyl isethionate (65%).
- Analytical purity: 66% (sodium acyl isethionate)
- Impurities (identity and concentrations): Free fatty acid (21%), sodium isethionate (7%)
- Composition of test material, percentage of components:
sodium acyl isethionate (66%)
Free fatty acid (21%)
sodium isethionate (7%)
water (2%)
misc. (4%)
- Purity test date: 1983-01-31
- Stability under test conditions: Stabillty of solid is good, can be stored at room temperature. In solution, at room temperature and neutral pH, solutions are stable, at hlgh temperature (greater than 50 degrees C) and low pH hydrolysis will occur to some extent.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: sewage microorganisms (source unspecified)

Details on inoculum:

No data (Check SOP)

Duration of test (contact time):

>= 28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

>= 10 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

>= 20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance 1

Reference substance:

acetic acid, sodium salt

Reference substance 2

Reference substance:

laurylsulfonate

Remarks:

Marlon A 350 (sodium salt)

Results and discussion

Preliminary study:

Not applicable

Test performance:

The extent of sodium acetate degradation (81.5%) shows that the inoculum used in the test was viable and therefore the test was valid.
Marlon A 350 failed the test (44.9% ultimate biodegradtion)

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

>= 77.8

Sampling time:

28 d

Remarks on result:

other: 10 mg/L concentration

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

>= 78.6

Sampling time:

28 d

Remarks on result:

other: 20 mg/L concentration

Details on results:

Defi (base) is readily and ultimately biodegradable. The level of biodegradation observed (77.8 and 78.6%) exceeded the pass level reccomended in OECD 301B. Greater than 60% degradation occured within 10 d of reaching 10% degradation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Defi (base) is readily and ultimately biodegradable. The level of biodegradation observed (77.8 and 78.6%) exceeded the pass level reccomended in OECD 301B. Greater than 60% degradation occured within 10 d of reaching 10% degradation.

Executive summary:

Defi (base) is readily and ultimately biodegradable. The level of biodegradation observed (77.8 and 78.6%) exceeded the pass level reccomended in OECD 301B. Greater than 60% degradation occured within 10 d of reaching 10% degradation.