Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 May 2011 - 11 July 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted to GLP in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Study design

HPLC method

Details on study design: HPLC method:
DETERMINATION OF DEAD TIME
The dead time was determined by measuring the retention time of formamide (purity* 99.94%, 728 mg/L solution in methanol:water (55:45 v/v)).

PREPARATION OF SAMPLE SOLUTION
Test item (0.0500 g) was diluted to 50 ml with acetonitrile.

REFERENCE SUBSTANCES
Solutions of reference standards (see table 1) were prepared in acetonitrile.

DETERMINATION OF RETENTION TIMES
The sample, dead time and reference standard solutions were injected in duplicate using the following HPLC parameters:
HPLC System: Agilent Technologies 1100, incorporating autosampler
Column: CN Optimal 5µm (150 x 4.6 mm id)
Column temperature: 35°C
Mobile phase: methanol:reverse osmosis water 55:45 v/v
pH of mobile phase: 6.7
Flow-rate: 1.0 ml/min
Injection volume: 10µl
UV detector wavelength: dead time and reference standards: 210 nm; sample: 240 nm

CONSTRUCTION OF CALIBRATION CURVE
A calibration curve was constructed from the retention time data of the deadtime and reference standard solutions. The capacity factors (k) for the reference standards were calculated using the equation below.

ADSORPTION COEFFICIENT OF SAMPLE
The capacity factor was calculated using the equation below and the log10Koc value determined using the equation with reference to the calibration curve.

Results and discussion

Adsorption coefficientopen allclose all
Type:
log Koc
Value:
4.17
Type:
Koc
Value:
14 600

Any other information on results incl. tables

Calibration

The retention times of formamide and the retention times, capacity factors (k) and log10Kocvalues for the reference standards are shown in the two following tables:

Dead Time

Retention Time (mins)

Mean Retention Time (mins)

Injection 1

Injection 2

Formamide

1.992

1.992

1.992

 

Standard

Retention Time (mins)

Mean

Retention

Time (mins)

Capacity

Factor (k)

 

Log10k

 

Log10Koc

Injection 1

Injection 2

Acetanilide

2.444

2.439

2.442

0.226

-0.647

1.25

Phenol

2.449

2.454

2.452

0.231

-0.637

1.32

Atrazine

2.760

2.760

2.760

0.386

-0.414

1.81

Isoproturon

2.974

2.974

2.974

0.493

-0.307

1.86

Triadinnenol

3.421

3.426

3.424

0.719

-0.143

2.40

Linuron

3.824

3.824

3.824

0.920

-3.64 x 10-2

2.59

Naphthalene

3.664

3.664

3.664

0.839

-7.61 x 10-2

2.75

Endosulfan-diol

4.461

4.466

4.464

1.241

9.37 x 10-2

3.02

Fenthion

5.272

5.243

5.258

1.639

0.215

3.31

α-Endosulfan

6.069

6.069

6.069

2.047

0.311

4.09

Phenanthrene

5.500

5.496

5.498

1.760

0.246

4.09

Diclofop-methyl

5.996

5.986

5.991

2.008

0.303

4.20

DDT

10.423

10.447

10.435

4.238

0.627

5.63

                                                                                          

 Adsorption coefficient of sample

The retention times, capacity factors and Log10Kocvalues determined for the sample are shown in the following table:

Injection

Retention Time(mins)

Capacity Factor (k)

Log10k

Log10Koc

1

6.152

2.09

0.320

4.17

2

6.142

2.08

0.319

4.16

 

Mean Log10Koc: 4.17

Adsorption coefficient:1.46 x 104

Discussion

The test determined an overall result for the isomeric forms of the test item which were considered not to vary significantly.

Applicant's summary and conclusion

Conclusions:
The adsorption coefficient (Koc) of the test item has been determined to be 1.46 x 10^4, Log10Koc 4.17.
Executive summary:

The adsorption coefficient (Koc) has been determined to be 1.46 x 104, log10Koc4.17 using the HPLC screening method, designed to be compatible with Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.