Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 May 2011 - 11 July 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted to GLP in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
5.09

Any other information on results incl. tables

Results

Preliminary estimate

The Log10Powwas calculated to be: 5.04

Definitive test

Calibration

The retention times of the deadtime and the retention times, capacity factors (k) and log10Powvalues for the reference standards are shown in the following tables:

Dead Time

Retention Time (mins)

Mean Retention Time

(mins)

 

Injection 1

Injection 2

Thiourea

1.599

1.589

1.594

 

Standard

Retention Time (mins)

Mean

Retention

Time (mins)

Capacity

Factor (k)

Log10k

Log 10 Pow

Injection 1

Injection 2

Benzene

3.605

3.596

3.601

1.26

0.100

0.3

Toluene

4.937

4.922

4.930

2.09

0.321

2.7

Naphthalene

6.088

6.074

6.081

2.80

0.449

3.6

Phenanthrene

11.759

11.740

11.750

6.37

0.804

4.5

Triphenylamine

27.094

27.046

27.070

16.0

1.20

5.7

DDT

38.455

39.387

38.421

23.1

1.36

6.5

 

Partition coefficient of sample

The retention times, capacity factors and log10Powvalues determined for the sample are shown in the following table:

Injection

Retention Time (mins)

 

Capacity Factor (k)

Log10k

Log10Pow

1

16.511

9.36

0.971

5.09

2

16.535

9.37

0.972

5.09

               

Mean log10Pow:5.09

Partition coefficient: 1.22 x 105

Discussion

Substances having a log10Pow greater than 3 are regarded as having the potential to bioaccumulate in the environment.

There was a peak seen at 17.4 minutes. This was probably a by-product. It had a log10Powof 5.18 and represented 7% of the test item by percentage area normalisation. The main peak in the chromatography used to determine the partition coefficient of the test item was 90% by area. The remaining approximately 3% comprised of several unidentified small peaks.

Applicant's summary and conclusion

Conclusions:
The partition coefficient of the test item has been determined to be 1.22 x 10^5, Log10 Pow 5.09.
Executive summary:

The partition coefficient of the test item has been determined to be 1.22 x 10^5, Log10Pow 5.09 using the HPLC method, designed to be compatible with Method A8 Partition Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.