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EC number: 403-030-6 | CAS number: 137398-54-0 ROBAC AS/100
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test substance attained betweeen 10 and 15% degradation after 28 days and cannot therefore be considered as readily biodegradable as defined within strict OECD Guidelines.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, not fulfilling specific criteria
Additional information
Method: Closed bottle test according to OECD Guideline No. 301D and EEC Directive 67/548 Annex V C6. as published in 84/449/EEC.
Duration: 28 days
Temperature: 21± 1ºC
Results:
Test concentration: 2 mg/l
Oxygen depletion (28 days): 0.375 mgO2/l
Theoretical oxygen demand (ThOD): 3.74 mg/O2/l
Percentage biodegradation (28 days): 10 -15%
Sodium benzoate (the standard reference substance) attained 92% degradation within 28 days.
Oxygen depletions in the inoculated and non-inoculated controls were within prescribed limits.
Method 2. Assessment of Ready Biodegradability, CO2 Evolution test OECD 301B- referenced as Method C.4 -C of Commission Regualtion (EC) no. 440/2008
Duration: 60 days
Temperature 20± 2ºC
Results:
Test concentration : 10mg/Carbon/L ( equivalent to 34.8 mg test item/L)
Biodegredation 15% after 28 days and 26% after 60 days
HPLC analysis, 65-67% of AS100 was recovered after 60 days. Recovery of hydrolysis product at 60days ranged between 7.44 and 7.83mg/L
Sodium Benzoate attained 87% biodegradation after 14 days, 88% after 28 days and 89% after 60 days therby confirming the suitability of the inoculum and test conditions
Validity of test:
1. Total Co2 evolution in the inoculum control vessel on Day 28 was 28.18mg/L - validation criteria satisfied
2. Inorganic Carbon (IC) content of the test item suspension in mineral medium at the start of the test was below 5% of the Total Carbon (TC) content
3. The difference between the values for CO2 production at the end of the test for the replicate vessels was <20% and hence satisfied the validation criterion given in the OECD test guidelines
Conclusion:
The test substance attained betweeen 10 and 15% degradation after 28 days and cannot therefore be considered as readily biodegradable as defined within strict OECD Guidelines.
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