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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
413-890-4
EC Name:
-
Cas Number:
82469-79-2
Molecular formula:
C28H50O8
IUPAC Name:
1,2,3-trihexyl 2-(butanoyloxy)propane-1,2,3-tricarboxylate

Test solutions

Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Agent has limited water solubility (0.1%). Stock solutions
were prepared at 5.26 mg/ml in DMF. Solutions were further
diluted to 3.16, 1.89, 1.14 and 0.68 mg/ml. These stocks
were delivered to the diluter and mixed with well water to
achieve final test concentrations.

Test organisms

Test organisms (species):
other aquatic crustacea: DM

Test conditions

Hardness:
148 mg CaCO3/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.38 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.2 mg/L
Details on results:
%Concentration loss over test: 14 ...

Applicant's summary and conclusion

Conclusions:
The EC50 for Daphnia magna = 0.380 mg/L/48 hr. The NOEL was 0.200 mg/L.