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Long-term toxicity to aquatic invertebrates

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Description of key information

The substance exhibited long-term adverse effects on Daphnia magna in a 21-day chronic aquatic toxicity test performed under semi-static conditions. The NOEC based on reproduction was 0.14 mg/L.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.14 mg/L

Additional information

The long-term toxicity of the submission item to aquatic invertebrates (Daphnia magna) was studied in a GLP long-term aquatic toxicity test in accordance with OECD TG 211 under semi-static conditions for a period of 21 days. The definitive test was performed using test concentrations of 0.014, 0.044, 0.14, 0.44 and 1.4 mg/L. The test material was dissolved in dimethylformamide and solvent stock solutions were then prepared, before the test solutions were adjusted by dilution in specially prepared culture medium. A control and a solvent control were run in parallel. Analytical confirmation of the nominal concentrations was carried out by measuring the test substance concentrations by means of HPLC. Especially the low measured concentrations were highly variable, but good agreement between nominal and measured concentrations was seen around the LOEC and the time-weighted mean measured concentrations (calculated from duplicate measured concentrations found in freshly prepared and expired test media) were in the range of 84 to 114% for the 0.14, 0.44 and 1.4 mg/L dose groups. The conditions of the test were such that reproduction was by parthenogenesis. Animals were taken from in-house laboratory cultures by separating young daphnids produced overnight. 10 healthy females each were distributed randomly over the testing vessels and controls.

Long-term exposure of aquatic invertebrates (Daphnia magna) to the test substance Gaskamine 240 resulted in significant mortalities at the test concentrations of 0.44 mg/L (60% after 21 days) and 1.4 mg/L (100% after 4 days). In addition, the reproductivity was significantly decreased in animals exposed to 0.44 mg/L. No effects (mortality, reproduction) were seen at lower test concentrations and the NOEC was 0.14 mg/L after 21 days of exposure.