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Diss Factsheets
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EC number: 700-786-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- The testing lab was not GLP in 2009 but study of good quality (klimisch score 2) so can be used for registration purpose
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23°C +/- 2
- pH:
- 7
- Nominal and measured concentrations:
- Test 1: 61.8, 121, 238.1, 475.6 and 1002.4 mg MS/L along with a control; Test 2: 19.7, 39.4, 78.8, 200.4 and 393.8 mg MS/L along with a control.
The test concentrations and effect results are expressed in mg of dry matter in the product per litre (mg MS/L, where MS = matière seche). - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 87 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Test 1
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 65 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Test 2
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Average of two tests.
- Details on results:
- CEr50-72h dry material : 76 mg/l
CEr50-72h raw material : 127 mg/l - Results with reference substance (positive control):
- CEr50-72h : 0.98mg/l
- Validity criteria fulfilled:
- yes
- Conclusions:
- CEr50-72h dry material : 76 mg/l (average of two tests)
Reference
Description of key information
Commercial samples of the registered substances are typically produced as aqueous solutions. An algae inhibition study was performed on a commercial quality of Sophorolipids (Lot 01/09), which had been determined to contain 60.6% dry matter. The % dry matter is equivalent to the Sophorolipid content of the test item.
Two tests were performed in the study, each using different concentration ranges, 61.8-1002.4 and 19.7-393.8 mg “dry matter” /L. The 72h ErC50 determined from both tests was similar (87 and 65mg/L based on dry matter). The average value of 76 mg “dry matter”/L has been chosen as the key value. A NOEC or EC10 was not determined.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 76 mg/L
Additional information
The effect of the registered substance (test item Sophorolipids, purity 60.6% dry matter) on the Growth of Freshwater Alga (Scenedesmus subspicatus) was followed over a period of 72 hours. The study was performed according to the European Standard ISO 8692 and OECD Guideline 201. Two tests were performed. The test concentrations and effect results are expressed in mg of dry matter in the product per litre (mg MS/L, where MS = matière seche).
The first test was performed at test concentrations of 61.8, 121, 238.1, 475.6 and 1002.4 mg MS/L along with a control. At the end of 72 hour observation period, 40.0%, 55.8%, 92.9%, 100% and 100% growth inhibition was observed in the test concentrations of 61.8, 121, 238.1, 475.6 and 1002.4 mg MS/L respectively. The 72h EC50 based on growth rate was calculated to be 87 mg MS/L.
The second test was performed at test concentrations of 19.7, 39.4, 78.8, 200.4 and 393.8 mg MS/L along with a control. At the end of 72 hour observation period, 12.1%, 19.0%, 59.2%, 95.9% and 100% growth inhibition was observed in the test concentrations of 19.7, 39.4, 78.8, 200.4 and 393.8 mg MS/L respectively. The 72h EC50 based on growth rate was calculated to be 65 mg MS/L.
The average 72 ErC50 from the two tests is 76mg MS/L.
A NOEC or EC10 was not determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.