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Toxicity to aquatic algae and cyanobacteria

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Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Reason / purpose:
reference to same study
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Analytical monitoring:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared following OECD guideline 23. A saturated stock solution was prepared.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): Collection of algal cultures in University of Goettingen, Germany
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23 - 23.2 °C
pH:
7.9 - 8.4
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
0, 1, 3.2, 10, 32, 100

Measured test concentration (mg/l):
at test start: n.d., nd. , 0.67, 2.22, 7.20
at 72 h: all test concentrations below detection limit
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml), gas permeable silicon sponge caps
- Fill volume: 100 ml
- Initial cells density: 50000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the OECD guideline 201


OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: universal white, average 5375 lux, wave length 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: chlorophyll-a-fluorescence at a wave length of 432 nm at 0, 24, 48 and 72 h.


TEST CONCENTRATIONS
- Range finding study: EC50 fluorescence: < 10 mg/l, ErC50. 13.5 mg/l
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
2.22 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 7.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Conclusions:
The acute toxicity of citronellyl acetate to Desmodemus subspicatus was tested in a guideline study according to OECD 201. At test termination after 72 hours an EC50 > 7.2 mg/l based on algal growth rate was detected. The concentrations was analytically determined (BASF SE, 2010).
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Justification for type of information:
See read across justification document attached in section 13.2
Reason / purpose:
read-across source
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Analytical monitoring:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared following OECD guideline 23. A saturated stock solution was prepared.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): Collection of algal cultures in University of Goettingen, Germany
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23 - 23.2 °C
pH:
7.9 - 8.4
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
0, 1, 3.2, 10, 32, 100

Measured test concentration (mg/l):
at test start: n.d., nd. , 0.67, 2.22, 7.20
at 72 h: all test concentrations below detection limit
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml), gas permeable silicon sponge caps
- Fill volume: 100 ml
- Initial cells density: 50000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the OECD guideline 201


OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: universal white, average 5375 lux, wave length 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: chlorophyll-a-fluorescence at a wave length of 432 nm at 0, 24, 48 and 72 h.


TEST CONCENTRATIONS
- Range finding study: EC50 fluorescence: < 10 mg/l, ErC50. 13.5 mg/l
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
2.22 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 7.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Conclusions:
The acute toxicity of citronellyl acetate to Desmodemus subspicatus was tested in a guideline study according to OECD 201. At test termination after 72 hours an EC50 > 7.2 mg/l based on algal growth rate was detected. The concentrations was analytically determined (BASF SE, 2010).

Description of key information

Acutely toxic for aquatic organisms

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
7.2 mg/L
EC10, LC10 or NOEC for freshwater algae:
2.22 mg/L

Additional information

The acute toxicity of citronellyl acetate to Desmodemus subspicatus was tested in a guideline study according to OECD 201. At test termination after 72 hours an EC50 > 7.2 mg/l based on algal growth rate was detected. The concentrations was analytically determined (BASF SE, 2010).