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Toxicity to aquatic algae and cyanobacteria

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Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
02 October 1995 to 05 October 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Test organisms (species):
Chlorella vulgaris
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23 °C
pH:
7.7 to 9.4
Nominal and measured concentrations:
Nominal concentrations of 0, 0.05, 0.10, 0.25, 0.50 and 1 mg/L test item
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.48 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.63 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Conclusions:
The test item gave EC50 (72 h) 0.48 mg a.i./L based on biomass and EC50 (72 h) 0.63 mg a.i./L based on growth rate of the freshwater species Chlorella vulgaris.
Executive summary:

The 72 hour EC50 of Lakeland ACP 70 to Chlorella vulgaris was estimated as 0.48 mg/L using the biomass integral and 0.63 mg/L by growth rate calculation. The 48 hour EC50 was 0.33 mg/L by biomass integral and 0.48 by growth rate. The values obtained indicate that at 72 hours there was no inhibition of algal growth or growth rate up to 0.25 mg/L. Inhibition calculated using both the biomass integral and the growth rate was significant at 48 hours at the lowest test concentration of 0.05 mg/I. The growth curves in Graph 1 show that at a concentration of 0.5 mg/L Lakeland ACP 70 growth was delayed, but a level of 1.0 mg/L almost completely stopped growth of the algae. The cell density of the control during the 72 hour test period increased by a factor of over 200, which is satisfactory.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
22 ± 2°C
pH:
- 7.37 to 8.10 at 0 h
- 7.12 to 7.67 at 72 h
Nominal and measured concentrations:
Nominal concentrations of 1.78, 3.16, 5.62, 10.0 and 17.8 mg/L prepared as water accomodated fractions.
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
1.91 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
< 1.78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Conclusions:
Results were reported as EC50 (24 h) 1.01 mg/L, EC50 (48 h) 1.51 mg/L and EC50 (72 h) 1.91 mg/L. It was not possible to calculate a NOEC value.
Executive summary:

A sample of LAKELAND ACP 70 was assessed for its potential toxicity to the freshwater alga Pseudokirschnereilla subcapitata in a static test over 72 hours according to the procedure described in OECD Guideline 201 Alga, Growth Inhibition Test and as detailed in STL Runcorn SOP 111.3. Water Accommodated Fractions (WAFs) containing concentrations of LAKELAND ACP 70, were prepared over the range 1.78 to 17.8 mg ri. Cultures of Pseudokirschnereilla subcapitata were exposed to determine the concentration which inhibited algal growth rate by 50% over 72 hours (ECSO). Results were reported as EC50 (24 h) 1.01 mg/L, EC50 (48 h) 1.51 mg/L and EC50 (72 h) 1.91 mg/L. The NOEC (72 h) value was reported as < 1.78 mg/L.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
20 ± 2 °C
pH:
- 8.11 to 8.49 (0 h)
- 8.24 to 8.35 (72 h)
Nominal and measured concentrations:
Nominal concentrations of ACP 70 of 0.1, 0.178, 0.316, 0.562, and l.0 mg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Conclusions:
Results were reported as EC50 (24 h) 0.38 mg/L, EC50 (48 h) 0.38 mg/L and EC50 (72 h) 0.40 mg/L). The NOEC value was reported as 0.1 mg/L.
Executive summary:

A sample of ACP 70 was assessed for its potential toxicity to the marine alga Skeletonema costatum in a static test over 72 hours. Nominal test concentrations of ACP 70, were prepared over the range 0.1 to 1.0 mg/L. Cultures of Skeletonema costatum were exposed to determine the concentration which inhibited algal growth rate by 50 % over 72 hours (EC50). Results were reported as EC50 (24 h) 0.38 mg/L, EC50 (48 h) 0.38 mg/L and EC50 (72 h) 0.40 mg/L). The NOEC value was reported as 0.1 mg/L.

Description of key information

Studies describing growth rate inhibition are available for freshwater and marine algae.

Key value for chemical safety assessment

EC50 for freshwater algae:
0.63 mg/L
EC50 for marine water algae:
0.4 mg/L
EC10 or NOEC for marine water algae:
0.1 mg/L

Additional information