Toluene-4-sulphonohydrazide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017.11.05-2017.11.27

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

17 July, 1992

Qualifier:

according to guideline

Guideline:

other: the National Institute of Environmental Research Guidelines (Biodegradation Test Method (Ready Biodegradability): Manometric Respirometry Test (Public Notice No. 2017-4))’.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identity CELLCOM-H
CAS No. 1576-35-9
KIT Code No. K-6920
Batch/Lot No. 201705001
Purity 99.27%
Storage conditions Room temperature
Expiry date May 15, 2018

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

The secondary effluent of a treatment plant (Jin-ju munsan public sewage treatment facility) was used as the inoculum. After collection, it was kept aerobic during transport and settled to
remove coarse particles for 1 hour. Then the inoculum was pre-conditioned by aerating for 5 days at the test temperature (22±2˚C) until required.

Duration of test (contact time):

28 d

Initial conc.:

50 mg/L

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

other: oxygen consumption

Value:

0

Sampling time:

28 d

Details on results:

The percentage microbial degradation of the test item by BOD meter was 0.0±0.0% at 28 days.

Results with reference substance:

The percentage microbial degradation of the reference item, sodium benzoate, by BOD meter was 74.4% at 14 days.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

CELLCOM-H, is not ready biodegradable according to OECD Guideline No 301F

Executive summary:

The purpose of the study was to evaluate the test item, CELLCOM-H, for its ready biodegradability in the aquatic environment. Biodegradation was studied according to ‘OECD Guidelines (Test No. 301 Ready Biodegradability, 301 F MANOMETRIC RESPIROMETRY TEST, (17 July 1992))’ and ‘the National Institute of Environmental Research Guidelines (Biodegradation Test Method (Ready Biodegradability): Manometric Respirometry Test (Public Notice No. 2017-4))’.

The percentage microbial degradation of the reference item, sodium benzoate, was 74.4% at 14 days The percentage microbial degradation of the test item by BOD meter was 0.0±0.0% at 28 days.

Therefore, the test item, CELLCOM-H, could not be classified as readily biodegradable

Description of key information

On the basis of a readily biodegradability study, the substance is concluded not to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

p-TSH is not readily biodegradable in the aquatic environment. In the ready biodegradability test with activated sludge (OECD TG 301F), the degree of biodegradation of this substance was found to be 0% after 28 days.