Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to aquatic invertebrates:

1. Based on the immobility of test organism daphnia magna due to the direct contact with the test chemical for 48 hrs, the effect concentration was 200 mg/l with the 95 % CI of 180 - 230 mg/L.

2. Based on the intoxication (Measured by observing the immobility) of daphnia magna by the exposure of chemical, the LC50 was determine to be 4730 mg/l.

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 200-4730 mg/l and not classified as per the CLP classification criteria.  

Toxicity to aquatic algae and cyanobacteria:

1. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to the direct contact with the chemical for 3 days, the effect concentration EC50 was 250 mg/l with the 95 % CL was 220 - 270 mg/L. and the NOEC was observed at the 16 mg/l.

2 .Based on the effect on Physiology and Assimilation efficiency of Chlorococcales (Green Algae Order), by the chemical, the EC50 was determine at 1300 mg/l.

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 250 -1300 mg/l and not classified as per the CLP classification criteria.  

Additional information

Short term toxicity to aquatic invertebrates:

Short term toxicity of (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

In the first study from authoritative database toxicity was measured. Aim of this study was to determine the acute Immobilization of test chemical to Daphnia magna for the exposure period of 48 hrs. Test conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure and the end of exposure. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by mixing and stirring the test substance and water. Test performed at different concentrations 350, 230, 160, 104, 69 mg/L (geometric ratio 1.5), control. Test vessel filled with 400 mL/ concentration (100 mL/vessel) contain 20 daphnia per concentration (5 / vessel). Experiment repeated 4 times with the same condition. Based on the immobility of test organism daphnia magna due to the direct contact with the chemical for 48 hrs, the effect concentration was 200 mg/l with the 95 % CI of 180 - 230 mg/L. Thus on the basis of EC50, chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.

 

Similarly another short term toxicity study from authoritative database 2018, was carried out for determination of the nature of chemical on the growth of aquatic invertebrates. Test conducted under the static system for the total exposure period of 24 hrs. <=24 h old daphnia magna were used in the study. Based on the intoxication (Measured by observing the immobility) of daphnia magna by the exposure of chemical, the LC50 was determine to be 4730 mg/l. Based on the LC50 chemical was concluded as nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 200-4730 mg/l and not classified as per the CLP classification criteria.  

Toxicity to aquatic algae and cyanobacteria:

Short term toxicity of (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0) on the growth and other biological activity of aquatic algae was carried out on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Study 1: Aim of this study was to determine the Growth Inhibition Test of Alga (Pseudokirchneriella subcapitata) by the test substance for the exposure period of 3 days. Test conducted according to OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test). Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure, after 4 hours from start of exposure, and at the end of exposure. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by adding and stirring the substance under the water surface of the Erlenmeyer flask filled with medium. Test performed on green algae Pseudokirchneriella subcapitata. Test was conducted at the different concentrations 500, 160, 50, 16, 5.0 mg/L (geometric ratio; square root of 10), control. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to the direct contact with the chemical for 3 days, the effect concentration EC50 was 250 mg/l with the 95 % CL was 220 - 270 mg/L. and the NOEC was observed at the 16 mg/l. Thus on the basis of EC50, chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.

 

Study 2: Toxicity study was carried out for determination of the nature of chemical on the growth of aquatic algae and cyanobacteria. Test conducted under the static system for the total exposure period of 24 hrs. Based on the effect on Physiology and Assimilation efficiency of Chlorococcales (Green Algae Order), by the chemical, the EC50 was determine at 1300 mg/l. Thus on the basis of EC50 value, chemical was concluded as nontoxic and not classified as per the CLP classification criteria. 

 

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 250 -1300 mg/l and not classified as per the CLP classification criteria.