4-[fluoro(dimethyl)silyl]butanenitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 21, 2016 - March 23, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted 13. Apr. 2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-[fluoro(dimethyl)silyl]butanenitrile

EC Number:

812-925-4

Cas Number:

1639345-42-8

Molecular formula:

C6H12FNSi

IUPAC Name:

4-[fluoro(dimethyl)silyl]butanenitrile

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The water-accommodated fractions (WAFs) were prepared for the test. This was done by weighing the nominal loads 4.6 / 10 / 22 / 46 / 100 mg/L (real load 4.6 / 10.2 / 22.3 / 46.0 / 100.7 mg/L) adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 μm nylon filters.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
-Species: Daphnia magna
-Variety: STRAUS
-Strain: Berlin
-Sex: female
-Age: between 0 and 24 hours
-Origin: Umweltbundesamt Berlin
-In-house breeding since: 27. September 2007

Husbandry
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical.
The husbandry is performed similar to the method described in the OECD guideline, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), Version 12
from 02. Feb. 2015.

-Vessels: preserving glasses, nominal volume 2 L
-Medium: M4-Medium (recipe of ELENDT)
-Food: unicellular green algae (Desmodesmus subspicatus)
- Medium renewal: twice a week
- Photo period: 16/8 hours, using neon tubes
-Temperature: 20 +/-2 °C

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250 in mg CaCO3/L

Test temperature:

19.1 – 21.0 °C

pH:

6.4 - 7.9

Dissolved oxygen:

The lowest concentration of dissolved oxygen at the end of the test was 8.8 mg/L

Salinity:

not applicable

Conductivity:

not given in the report

Nominal and measured concentrations:

The measured concentrations were in a range of 89 % - 110 % of the nominal concentration. Therefore, the determination of the results was based on the mean of the nominal concentrations.

Details on test conditions:

TEST SYSTEM

- Test vessel:

- Type: open

- Material, size, headspace, fill volume: Beakers, glass, nominal volume 50 mL

- Aeration: no

- No. of organisms per vessel: 5

- No. of vessels per concentration (replicates): 4

- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Deionised water with an enrichment of certain minerals (dilution water as demanded in the guidelines) was used in the test.


OTHER TEST CONDITIONS

- Adjustment of pH: no

- Photoperiod: 16 h light, 8 h dark


EFFECT PARAMETERS MEASURED: Mobility after 24 and 48 h.

TEST CONCENTRATIONS

- concentrations applied: 4.6 / 10 / 22 / 46 / 100 mg/L (nominal concentrations)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

but exposure is to hydrolysis products

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

but eyposure is to hydrolysis products

Basis for effect:

mobility

Details on results:

- Mortality of control: 0

Results with reference substance (positive control):

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study. The 24h-EC50 value was determined as 1.7 mg/L, lying within the demanded range of 0.6 – 2.2 mg/L stated in the guideline.

Reported statistics and error estimates:

There were no toxic effects observed in the test and therefore statistical analysis of the results was not required.

Any other information on results incl. tables

Table 1 Calculated Test item Concentrations

 

Nominal Concentration Test Item	Calculated Concentration Test Item t = 0 h	Calculated Concentration Test Item t = 48 h	% of Nominal concentration t = 0 h	% of Nominal concentration t = 48 h
mg/L	mg/L	mg/L	%	%
Blank control	--	--	--	--
4.6	4.9	4.1	106	89
10	9.4	10.5	94	105
22	21.7	23.4	99	106
46	46.0	49.6	100	108
100	101.6	110.1	102	110

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A 48-hour EC50 value of >100 mg/L and a NOEC of ≥100 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna based on nominal exposure concentrations of the substance. The test substance hydrolyses in water and it is therefore likely that the test organisms were primarily exposed to the hydrolysis products of the substance.