1-Methyl-4-[(trans,trans)-4'-propyl[1,1'-bicyclohexyl]-4-yl] -benzene

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-09-20 to 2005-12-05

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: METI Guideline: Concentration Test on Chemical Substances in Fish

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 28
- Sampling intervals/frequency for test medium samples: days 0, 7, 14, 21, 28
- Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane. Further analysis performed by HPLC and GC.

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 5 g/l
Dose 1: Test item: 0.2 mg/L , HCO-40: 10 g/L
Dose 2: Test item: 0.02 mg/L, HCO-40: 10 g/L
Control: Test item: --, HCO-40: 10 g/L

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Weight: 6.6 g
Length: 8.3 cm
Lot: 050714c

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

0

Test temperature:

24.6 - 25.0 °C

pH:

7.3-7.4

Dissolved oxygen:

saturated

Salinity:

According to guideline

Details on test conditions:

After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 7, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.20 and 0.02 mg/L were maintained during the study (0.2mg/L nominal: 0.194 - 0.199 mg/L; 0.02 mg/L nominal: 0.0194-0.0202 mg/L).At days 7, 14, 21, 25 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted with acetonitrile and n-hexane. The extract was analysed with HPLC.Due to the low bioaccumulation potential, no depuration phase was required.

Nominal and measured concentrations:

nominal concentration 0.5 mg/L:
analytically measured concentrations:
day 4: 0.199 mg/L
day 7: 0.196 mg/L;
day 14: 0.194 mg/L
day 21: 0.198 mg/L
day 28: 0.196 mg/L

nominal concentration 0.05 mg/L
analytically measured concentrations:
day 4: 0.0200 mg/L
day 7: 0.0202 mg/L
day 14: 0.0194 mg/L
day 21: 0.0201 mg/L
day 28: 0.0195 mg/L

Details on estimation of bioconcentration:

according to guideline

Key result

Conc. / dose:

0.2 mg/L

Type:

BCF

Value:

40 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

7 d

Calculation basis:

steady state

Key result

Conc. / dose:

0.02 mg/L

pH:

7

Type:

BCF

Value:

38 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

7 d

Calculation basis:

steady state

Details on kinetic parameters:

steady state was reached within 7 days. Due to the low uptake, no depuration phase was necessary

Metabolites:

none

Details on results:

- Mortality of test organisms: no effects
- Behavioural abnormalities:no effects
- Observations on body length and weight:no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects

Validity criteria fulfilled:

yes

Conclusions:

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 39. The test material showed no relevant bioaccumulation (BCF < 2000).

Executive summary:

Purpose

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions.

Study Design

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.2 and 0.02 mg/L were used in this study. HCO-40 was used as vehicle at concentrations of 10 g/L. The exposure was maintained during the couse of the study at about 98 -100 % of the nominal concentrations. The concentrations have been verified analytically using a LC method.

Results

No signs of toxicity were observed throughout the study. The analysis reveals a steady state BCF of 39 (38, 40). Due to the low bioaccumulation potential, no depuration phase was required.

Conclusion

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF value was at 39.

Description of key information

The steady state BCF values were at 39 thus the test material showed no relevant bioaccumulation (BCF < 2000).

Key value for chemical safety assessment

BCF (aquatic species):

39 dimensionless

Additional information

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.2 and 0.02 mg/L were used in this study. HCO-40 was used as vehicle at concentrations of 10 g/L. The exposure was maintained during the couse of the study at about 98 -100 % of the nominal concentrations. The concentrations have been verified analytically using a LC method.

No signs of toxicity were observed throughout the study. The analysis reveals a steady state BCF of 39 (38, 40). Due to the low bioaccumulation potential, no depuration phase was required.

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF value was at 39.