Registration Dossier
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EC number: 233-701-7 | CAS number: 10319-14-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Within 28 days, a degradation rate of 0 % was determined.
Similar substance 1 is considered to be "Not Readily Biodegradable".
A detailed description of the read across chosen is reported in section 13.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The test for the biodegradability potential was performed on similar substance 1 according to EU method C.4 -D “Manometric Respirometry Test“(2008) and the OECD guiedeline 301 F (adopted July 1992).
The substance was incubated for 28 days in continuously stirred 250 mL closed flask (in duplicate) in the dark with an inoculum originally collected from local, predominantly municipal wastewter treatment plant. In this assay, biodegradation was estimated by biological oxygen demand (BOD) over time. BOD was measured daily by a manometer. The incubation temperature was 22 ± 1 °C, pH was 7.4 - 8.0. The concentration of inoculum was 30 mg/L and the one of the test substance was a suspension of 100 mg/L. Degradation was calculated by substracting the amount BOD in the negative (inoculum only) control from that in the substance or positive control at any given time point and divided by the chemical oxygen demand (COD) or thereotical oxygen demand (ThOD). The 28 -days degradation was 0 % and for the positive control (with reference substance) the biodegradation was 88 %. Therefore, the test substance is considered to be “Not Readily Biodegradable“.
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