Registration Dossier
Registration Dossier
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EC number: 204-340-2 | CAS number: 119-64-2
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- The cytotoxicity of some organic solvents
- Author:
- Holmberg B and Malmfors T
- Year:
- 1 974
- Bibliographic source:
- Environ. Res. 7, 183-192
Materials and methods
- Type of method:
- in vitro
Test material
- Reference substance name:
- 1,2,3,4-tetrahydronaphthalene
- EC Number:
- 204-340-2
- EC Name:
- 1,2,3,4-tetrahydronaphthalene
- Cas Number:
- 119-64-2
- Molecular formula:
- C10H12
- IUPAC Name:
- 1,2,3,4-tetrahydronaphthalene
Constituent 1
Test animals
- Details on test animals or test system and environmental conditions:
- Ehrlich-Landschütz diploid (ELD) ascites tumour cells propagated in outbred albino NMRI mice
Administration / exposure
- Details on exposure:
- TC-Freetext:
- Test vessel: sealed 3 ml glass tubes filled to the top
- Test concentrations: 25, 50, 75, or 100 ppm (= mg/l)
- Test temperature: 37 +/- 1 degree C
- Aeration: No; constant stirring
- No. of replicates: one per concentration and observation
- Observation: after 0, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5 h
- Evaluation: counting of 200 cells per sample after staining with 2 % Lissamine green B
- Details on study design:
- Cytotoxicity of tetrahydronaphthalene was studied in Ehrlich-Landschütz diploid (ELD) ascites tumour cells propagated in outbred albino NMRI mice.
Results and discussion
Any other information on results incl. tables
RS-Freetext:
Percent of irreversibly injured cells:
------------------------------------------------------
Time 0 mg/l 25 mg/l 50 mg/l 75 mg/l 100 mg/l
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0 h 2.3 3.0 3.0 3.0 4.0
1 h 2.6 2.5 5.5 4.5 11.0
2 h 3.1 6.5 15.0 17.0 41.5
3 h 3.6 8.0 16.0 17.5 40.5
4 h 3.5 15.0 18.0 24.5 67.0
5 h 4.2 ? 18.5 ? 95.5
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1,2,3,4-tetrahydronaphthalene was assigned a high toxicity towards ELD cells.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
