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EC number: 203-678-8
CAS number: 109-53-5
The keratinocyte activating potential of test substance
Isobutylvinylether was evaluated in the LuSens assay. For this purpose,
the test substance was incubated with a luciferase reporter cell line
(LuSens cells) for ca. 48 hours at 37°C and antioxidant response element
(ARE) dependent luciferase activity was measured in a luminometer.
In order to determine the concentrations suitable for the main
experiment a pre-test (non-GLP) was performed. Cells were exposed to
several concentrations of the test substance and cytotoxicity was
determined by MTT assay. No cytotoxicity was observed.
In the main test luciferase activity was measured after 48-hour
exposure. In parallel a MTT assay was performed to assess cytotoxicity
of the test substance. A total of 2 valid experiments were performed.
The following results were observed:
At concentrations used in the main experiment the test substance was
soluble in DMSO (100 x stock preparations) and in 1% DMSO in culture
medium 3 (final concentrations). No precipitates were noticed in any
concentration after 48 hours.
In summary, after 48 hours of exposure to test substance
Isobutylvinylether luciferase activity in LuSens cells was not induced
in at least two consecutive concentrations with statistical significance
affording at least 70% viability in at least two independent
experiments. From this it has to be concluded that test substance
Isobutylvinylether does not have a keratinocyte activating potential.
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