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Acute tests on all three trophic levels were performed to examine the aquatic toxicity of isobutyl vinyl ether. Fish turned out to be the most sensitive species revealing a LC50 (96h) of 28.3 mg/L. Effects towards invertebrates and daphnia were found in the same range providing an ErC50 (72h) of 45.9 mg/L and an EC50 (48h) of 52 mg/L, respectively. Thus, isobutyl vinyl ether is considered acutely harmful for aquatic organisms.