1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Jan to March 1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

EC Number:

250-961-7

EC Name:

1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

Cas Number:

32220-82-9

Molecular formula:

C41H27N7O6

IUPAC Name:

1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]

Test material form:

other: mikrodispers

Details on test material:

Cibanon Blau 83962 Mikrodispers

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Zebrafish: Danio rerio
Replicates: 10 per test concentration
Age: about 4-6 months
Size:3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg.

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 21-25 °C.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Not available

Test temperature:

23°C

pH:

7.5 to 7.7

Dissolved oxygen:

7.7 to 8.0

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 100, 300 and 1000 mg/L

Details on test conditions:

Water:
- 3 liters for Zebra fish (active coal filtered), Water used according to ISO guidelines.

Basins:
- Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

589.49 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

462.39 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- At 100 and 300 mg/L, all fishes survived after 48 and 96 hours of exposure time.
- At 1000 mg/L, all fishes survived until 4 hours of exposure time and all died after 24 hours of exposure time.

Applicant's summary and conclusion

Conclusions:

The LC50 of the test substance in this fish acute toxicity test was determined to be 589.49 mg/L and 462.39 after 48 and 96 hours exposure time, respectively.

Executive summary:

The acute fish toxicity test was performed according to Method A-11.

10 Zebra fish were used in this test at each dose level.

The fish were tested at these differents follwing concentrations: 100, 300 and 1000 mg/L as in the pre-test all fishes survived at 100 mg/L.

- At 100 and 300 mg/L, all fish survived after 48 and 96 hours of exposure time.

- At 1000 mg/L, all fish survived until 4 hours of exposure time and all died after 24 hours of exposure time.

Results:

After 48 hours exposure time:

LC0 =300 mg/L

LC50 = 589.49 mg/L

LC100 =1000 mg/L

After 96 hours exposure time:

LC0 =300 mg/L

LC50 462.39 mg/L

LC100 =1000 mg/L

The LC50 of Vat Blue 66 in this fish acute toxicity test was determined to be 589.49 mg/L and 462.39 mg/L after 48 hours and 96 hours exposure time, respectively.