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EC number: 220-974-2 | CAS number: 2955-37-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 7-chloro-5-(2-chlorophenyl)-1,3-dihydro-2H-benzo-1,4-diazepin-2-one 4-oxide
- EC Number:
- 220-974-2
- EC Name:
- 7-chloro-5-(2-chlorophenyl)-1,3-dihydro-2H-benzo-1,4-diazepin-2-one 4-oxide
- Cas Number:
- 2955-37-5
- Molecular formula:
- C15H10Cl2N2O2
- IUPAC Name:
- 7-chloro-5-(2'-chlorophenyl)-1,3-dihydro-2H-1,4-benzodiazepin-2-one 4-oxide
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- During the final test duplicate samples for analysis were taken from the filtrate and the control.
Sampling: Frequency: at t=0h and t=48h.
Volume: 10 ml from the approximate centre of the test vessel.
Storage: All samples were stored in a freezer.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The test solutions were prepared using stock solutions of 100 mg/L by adding quantitatively 100 mg of test substance to 1000 ml ISO-medium. The resulting dispersion was treated with ultrasonic waves for 5 minutes (final study) and magnetically stirred for 3 days (both tests), after which it was ahazy solution with test substance particles, a precipitate and a floating layer. The solution was then filtered through a paper filter (ca. 5 µm, Schleicher and Schuell) to remove the larger undissolved particles. The resulting solution was clear and colourless. Lower test concentrations were prepared by subsequent dilution in test medium (range-finding test). All final solutions were clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Daphnia magna (Straus 1820)
- Source: in-house al culture
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 19.9-20.3
- pH:
- 7.9-8.0
- Dissolved oxygen:
- 8.6-9.0 mg/L
- Nominal and measured concentrations:
- limit test: 100 mg/L
range-finding test: 0.1, 1.0, 10 and 100 mg/L - Details on test conditions:
- Test vessels: 100 ml, all-glass
Medium: ISO, prepared in milli-RO water
Number of daphnia: 20 per concentration
Loading: 5 per vessel containing 80 ml medium
Light: 16 hours photoperiod daily
Feeding: No feeding
Aeration: No aeration of the test solutions.
lntroduction of daphnia: Within 3/4 hours after preparation of the test solutions. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Remarks:
- loading rate
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 12 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- LNO did not induce acute immobilisation of Daphnia magna at a loading rate of 100 mg/L (NOELR), corresponding to an actual concentration of 12 mg/L (NOEC).
- Results with reference substance (positive control):
- The reference test was carried out to check the sensitivity of the test system as used by NOTOX. Daphnia were exposed for a maximum of 48 hours to K2Cr2O7 concentrations of 0.1, 0.18, 0.32, 0.56, 1.0 and 1.8 mg/L and to a blank control. Twenty daphnia were exposed per concentration. The 24h-EC50 was 1 .0 mg/L with a 95% confidence interval between 0.94 and 1.2 mg/L. The 48h-EC50 was 0.74 mg/L with a 95% confidence interval between 0.66 and 0.84 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study LNO did not induce acute immobilisation of Daphnia magna at a loading rate of 100 mg/L (NOELR), corresponding to an actual concentration of 12 mg/L (NOEC).
The EL50 was above a loading rate of 100 mg/L. The EC50 was above the maximum soluble concentration in test medium (> 12 mg/L).
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