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Toxicity to aquatic algae and cyanobacteria

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Description of key information

The test item showed no effects up to the limit of water solubility and beyond.

Key value for chemical safety assessment

Additional information

The toxicity of to the unicellular freshwater green alga Pseudokirchneriella subcapitatawas determined according to the principles of OECD 201, Commission Regulation (EC) No, 761/2009, C.3 (2009) and EPA Guideline 712-C-006: OCSPP 850.4500 (2012).

The aim of the study was to assess the effects on biomass integrals, growth rate and yield over a period of 96 hours.

The study was conducted under static conditions with an initial cell density of 10010 cells/mL. The saturated solution and further four dilution levels were tested in a geometrical series with a dilution factor of 2:6.25 - 12.5 - 25.0 - 50.0-100 % of the saturated solution, corresponding to the nominal concentrations 0.313 - 0.625 - 1.25 - 2.50 - 5.00 mg/L.

Four replicates were tested for each test item concentration and eight replicates for the control. The environmental conditions were within the acceptable limits.

No recovery phase was performed, because no concentration level was inhibited by more than 50 %.

The concentrations of the test item in all test concentrations and the controlwere analytically verified via HPLC-UV at the start and the end of exposure. The recovery rates of the test item at the start were in a range between 10 % and below the limit of quantification. At the test end, the recovery values ranged from 5 % to below the limit of quantification.

All effect values were based on the nominal concentrations of the test item. In this study, the substance was found not to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata with the following effect values (nominal test item concentrations): The EC50-values for inhibition of biomass (EbC50), growth rate (ErC50) and yield (EyC50) after 72 and 96 hours were > 5.00 mg/L. The NOEC-values for inhibition of biomass, growth rate and yield after 72 and 96 hours were 5.00 mg/L.