Reaction product of 3-[4-(2-aminoethylamino)-6-(4-sulfoanilino)-1,3,5-heterocycle-2-ylamino]benzensulfonic acid, ammonia water, sodium chloride and {mixture of [poly(1~4)chlorosulfonylphthalocyaninato-N29,N30,N31,N32]copper(II), [poly(1~3)chlorosulfonyl (tribenzo[b,g,l]pyrido[2,3-q]-5,10,15,20-tetraazaporphyrinato-N21,N22,N23,N24)]copper(II),[poly(1~2)chlorosulfonyl(dibenzol[b,g(or b,l)dipyrido[2,3(or 3,2)-l,q (or g,q)]-5,10,15,20-tetraazaporphyrinato-N21,N22,N23,N24)] copper(II) and [monochlorosulfonyl(benzo[b]tripyridol[2,3(or 3,2)-g,lq]-5,10,15,20- tetraazaporphyrinato- N21,N22,N23,N24)] copper(II)

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 January 2009 to 07 February 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

yes

Remarks:

Please see below.

Principles of method if other than guideline:

The method guideline states that the measurement of adsorption coefficient should be carried out on substances in their ionised and unionised forms. However, the dissociation constants of certain functional groups found in the test material made it impossible to satisfy this criteria. Testing would be required at pH <0 and at pH >10 for the acidic and basic functional groups respectively. As a consequence of this, one of the functional groups will always be present in its ionised form. Therefore, as these pH’s are outside the environmental pH range for this test and also experimentally impractical, testing was carried out at approximately neutral pH with the test material being ionised. pH 7 is also the recommended pH for salts of sulphonic acid groups.

GLP compliance:

yes (incl. QA statement)

Remarks:

Date of inspection: 19 August 2008; Date of signature: 04 March 2009

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Sponsor's identification : H-CB sodium salt
Description : blue solid
Purity : 94.8%
Lot number : MB-2
Date received : 04 December 2008
Storage conditions : room temperature in the dark

Radiolabelling:

not specified

Study design

Test temperature:

Not stated.

Batch equilibrium or other method

Analytical monitoring:

yes

Results and discussion

Adsorption coefficient

Key result

Type:

log Koc

Value:

1.25 dimensionless

pH:

7

Temp.:

20 °C

Any other information on results incl. tables

Calibration

The retention times of formamide and the retention times, capacity factors (k) and log₁₀ K_oc values for the reference standards are shown in the two following tables:

Table 2

Dead Time	Retention Time (mins)		Mean Retention Time (mins)
	Injection 1	Injection 2
Formamide	3.381	3.376	3.379

Table 3

Standard	Retention Time (mins)		Mean Retention Time (mins)	Capacity Factor (k)	Log10k	Log10Koc
	Injection 1	Injection 2
Acetanilide	4.075	4.077	4.076	0.206	-0.685	1.25
Phenol	4.084	4.083	4.084	0.209	-0.681	1.32
Atrazine	4.514	4.507	4.511	0.335	-0.475	1.81
Isoproturon	4.901	4.902	4.902	0.451	-0.346	1.86
Triadimenol	5.276	5.277	5.277	0.562	-0.250	2.40
Linuron	6.158	6.157	6.158	0.823	-8.48 x 10^-2	2.59
Naphthalene	7.900	7.900	7.900	1.34	0.127	2.75
Endosulfan-diol	6.090	6.094	6.092	0.803	-9.52 x 10^-2	3.02
Fenthion	10.373	10.377	10.375	2.07	0.316	3.31
a-Endosulfan	10.571	10.581	10.576	2.13	0.328	4.09
Phenanthrene	14.078	14.053	14.066	3.16	0.500	4.09
Diclofop-methyl	12.756	12.759	12.758	2.78	0.443	4.20
DDT	30.893	30.959	30.926	8.15	0.911	5.63

 

Adsorption coefficient of sample

The retention times, capacity factors and log₁₀ K_oc values determined for the sample are shown in the following table:

Table 4

Injection	Retention Time (mins)	Capacity Factor (k)	Log10k	Log10Koc
1	1.687	<0.206	<-0.685	<1.25
2	1.686	<0.206	<-0.685	<1.25

Mean log₁₀ K_oc:                     <1.25
Adsorption coefficient:        <17.8

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient (Koc) of the test material has been determined to be <17.8, log10 Koc <1.25.

Executive summary:

The Adsorption Coefficient was calculated to be less than 17.8, log₁₀ K_oc <1.25 using the HPLC screening method, Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.