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EC number: 277-753-9
CAS number: 74186-17-7
Results after treatment with
Aluminium-Braun 1134 p and the controls (60 minutes exposure
absorbance per tissue [rounded values]
absorbance per treatment group [rounded values]
vitro study was performed to assess the irritation potential of the
test item by means of the Human Skin Model Test.
test item passed the MTT interference pre-test. Due to its intensive
colour, an additional test with one viable tissue (without MTT addition)
was necessary to correct the result in the main experiment.
25 mg of the test item were applied to each tissue, wetted with 25 µL of
DPBS, and spread to match the surface of triplicate tissue.
of either the negative control (DPBS) or the positive control (5% SLS)
were applied to triplicate tissue each.
test item and the positive and negative controls were washed off the
skin tissues after 60 minutes treatment. After further incubation for
about 42 hours the tissues were treated with the MTT solution for 3
hours following 69 hours extraction of the colorant from the cells. The
amount of extracted colorant was determined photometrically at 570 nm.
treatment with the negative control the absorbance values were well
within the required acceptability criterion of mean OD³ 0.8
and ≤ 2.8 for the 60 minutes treatment interval thus showing the quality
of the tissues.
with the positive control induced a decrease in the relative absorbance
as compared to the negative control to 4.5% thus ensuring the validity
of the test system.
relative standard deviations between the % variabilities of the test
item, the positive and negative controls in the main test were below 7%
(threshold of the "OECD Guideline for the Testing of Chemicals 439:In
vitroSkin Irritation: Reconstructed Human Epidermis Test Method”: <
18%), thus ensuring the validity of the study.
Compared to the relative absorbance
value of the negative control the mean relative absorbance value was
reduced to 80.5% (corrected value) after exposure of the skin tissues to
the test item. This value is above the threshold for irritancy of ≤ 50%.
Therefore, the test item is not considered to possess an irritant
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