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Short-term toxicity to fish:

Various studies for the target chemical 4-Aminoazobenzene-3, 4’-disulfonic acid (Cas no.101-50-8) along with read across substance data were reviewed to summarize the following information:

96 hrs aquatic toxicity study (SSS QSAR prediction model, 2016) was conducted to assess toxic effects of the test compound 4-Aminoazobenzene-3, 4’-disulfonic acid (CAS no 101 -50 -8) and the results were predicted. This predicted study indicate that the short term toxicity (LC50) in Brachydanio rerio (new name: Danio rerio) fish to be 291.161651611 mg/l in static condition.

Other read across substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (Cas no. 2706-28-7)study from German GSBL database;2016 indicate that the lethal concentration (LC0) value was obtained be to 1000 mg/l for 48 hrs.on mortality, immobilization and Cell proliferation inhibition effects.

On the basis of the above results of predicted data for target chemical and its read across substance, it can be concluded that the substance can be classified as non- hazardous to fish as per the criteria of CLP regulation.

Toxicity to aquatic algae and cyanobacteria

Various predicted data for the target chemical4-Aminoazobenzene-3,4'-disulfonic acid (CAS no. 101-50-8) were reviewed to summarize the following information:

 

72 hrs aquatic toxicity study (SSS QSAR prediction model, 2016) was predicted to assess toxic effects of the test compound 4 -Aminoazobenzene-3,4'- disulfonic acid (CAS No. 101 -50 -8) and the results were predicted. The study was based on the effects of the test compound on Desmodesmus subspicatus in a static fresh water system. The predicted data suggests the effective concentration (EC50) for the test compound 4-Aminoazobenzene-3,4'-disulfonic acid (CAS No. 101 -50 -8) was estimated to be 128.468963623 mg/L on the basis of growth rate.

 

96 hrs aquatic toxicity study (EPI suite, 2016) waspredictedto assess toxic effects of the test compound4-Aminoazobenzene-3,4'-disulfonic acid (CAS no. 101-50-8)and the results were predicted. The study was based on the effects of the test compound on green algae in a static fresh water system. The predicted data suggests the effective concentration (EC50) for the test compound4-Aminoazobenzene-3,4'-disulfonic acid (CAS no. 101-50-8)was estimated to be 12858.936 mg/l on the basis of growth rate.

 

72 hrs aquatic toxicity study (Danish (Q)SAR Database, 2016) was predicted to assess toxic effects of the test compound 4 - Aminoazobenzene-3,4'- disulfonicacid (CAS no. 101-50-8)and the results were predicted. The study was based on the effects of the test compound on Pseudokirchneriella spp. in a static fresh water system. The predicted data suggests the effective concentration (EC50) for the test compound4-Aminoazobenzene-3,4'-disulfonic acid (CAS no. 101-50-8)was estimated to be 2749.327mg/L on the basis of growth rate.

 

 

 

On the basis of the above resultsof predicted data for target chemical, it can be concluded thatthe substance can be considered as non-toxic to aquatic organisms and thus, cannot be classified as hazardous as per the criteria of CLP regulation.

Short term toxicity to aquatic invertebrates:

From predicted studies for the target chemical 4-aminoazobenzene-3,4'-disulphonic acid along with its read across substance were reviewed to summarize the following information: 

48 hrs aquatic toxicity study (EPI suite, 2016) was estimated to assess toxic effects of the test compound 4-aminoazobenzene-3,4'-disulphonic acid (CAS no 101-50-8) and the results were predicted. The predicted study was based on the effects of the test compound on Daphnia magna in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for the test compound 4-aminoazobenzene-3,4'-disulphonic acid was estimated to be147.616073608mg/L on the basis of mobility. 

 

In a short term toxicity study,(M. St. J. Warneand A. D. Schifko )Ceriodaphnia dubia exposed to Tartrazine (1934 -21 -0)in the nominal concentration of 4932 to 6602 ug/L for 48 hours in semi-static fresh water condition. Test was conducted in five concentrations with triplicated. Immobilization, protease, lipase, a-amylase, and cellulase enzymes were examined and mortality was calculated from molecular weight. No Immobilization and 50 % mortality was observed at 5706.55 mg/l with 95% CL level 4932 - 6602mg/l. Therefore, EC50 was determined to be 5706.55 mg/l when Ceriodaphnia dubia exposed to Tartrazine for 48 hours.

Short term toxicity to aquatic invertebrates (GSBL web application) by Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate were performed on Daphnia Magna for 48 hrs at 1000 mg/l concentration.After experiment it was determined that the EC0 value for Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (2706-28-7) to Daphnia magna was observed to be 1000 mg/l on the basis of mortality,immobilization and cell proliferation inhibition.

On the basis of results of various predictions and studies of read across for toxicity to aquatic invertebrates, it can be considered that the substance is not likely to be toxic to aquatic invertebrates at environmentally relevant concentrations and also can be classified as non- hazardous as per the criteria of CLP regulation.

Toxicity to microorganisms:

Various studies for the target chemical 4-Aminoazobenzene-3, 4’-disulfonic acid (Cas no.101-50-8) along with read across substance data were reviewed to summarize the following information:

48 hrs aquatic toxicity study (SSS QSAR prediction model, 2016) was conducted to assess toxic effects to micro-organism of the test compound 4-Aminoazobenzene-3, 4’-disulfonic acid (CAS no 101-50-8) and the results were predicted. This predicted study indicate that the toxicity to micro reganism (IGC50) in Tetrahymena pyriformis to be 921.177mg/l.

Other read across substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (Cas no. 2706-28-7)study from German GSBL database;2016 indicate that the effect concentration (EC0) value was obtained be to 5000 mg/l for 24 hrs.on Mortality, swimming behaviour and Cell proliferation inhibition effects.

On the basis of the above results of predicted data for target chemical and its read across substance, it can be concluded that the substance can be classified as non- hazardous to micro organism.