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EC number: 215-089-3
CAS number: 1300-71-6
Table 1: Male and female combined data -
24hr time point
CPA - cyclophosphamide
VIN - Vincristine
2: Male and female combined data - 48hr time point
a bone marrow micronucleus assay using NMRI mice, a single oral gavage
of 3,5-xylenol was administered to groups of male and female animals,
employing a dose volume of 10 mL/kg. Doses were selected from a pilot
toxicity study doses of 1500 and 1750 mg/kg/bw were administered. Mortality
was observed in at 1750mg/kg and evident signs of toxicity were observed
at 1500mg/kg. The MTD was therefore deemed to be 1500 mg/kg.
control groups were treated with vehicle only (olive oil) and positive
control groups were treated with the clastogen, cyclophosphamide (CPA,
20 mg/kg bw) or the aneugen, vincristine (0.15 mg/kg). Mouse
bone marrow was sampled at 24 and 48 hours after dosing for the vehicle
and 3,5 xylenol dosed groups. A
single sampling time of 24 hours after dosing was used for both positive
control groups. Slides
of bone marrow cells were prepared from five animals/sex/time point for
each group and scored for the occurrence of micronucleated polychromatic
erythrocytes (MN PCE) and PCE/total erythrocyte ratios.
(1 male and 1 female) at 1500mg/kg, 48 hr sample point were observed.
Clinical signs of toxicity included irregular breathing (375mg/kg).
squatting posture (750mg/kg) and piloerection and squatting (1500mg/kg).
There were no marked decreases in mean PCE/total erythrocyte ratio
observed for any of the 3,5 xylenol treated groups compared to the
vehicle control group for either time point.
of the mean MN PCE group data indicated that there was no statistically
significant increases MN PCE frequency compared to concurrent control
values for either sex. Indiviudaul animal and group mean MN PCE
frequencies were consistent with both the concurrent vehicle control
values and the historical control. Positive
control treatment induced the appropriate response.
analysis confirmed the suitability of the doses prepared.
is concluded that 3,5 xylenol did not induce micronuclei in the
polychromatic erthrocytes of the bone marrow following sampling at 24
and 48 hours post dosing of both male and female rats when tested at a
dose of 1500 mg/kg (deemed a maximum tolerated dose) under the
conditions of the assay described.
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