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EC number: 237-529-3
CAS number: 13826-66-9
The endpoint is covered by a study performed with zirconium dinitrate
oxide (Sipos, 2015) reporting a 96-h LL50 > 100 mg/L (anhydrous
zirconium dinitrate oxide) for rainbow trout. In this study, no
measurable dissolved zirconium levels could be obtained, even at a pH of
6.0, which was considered as the lowest pH feasible for testing with
rainbow trout. Based on these findings, zirconium dinitrate oxide is
concluded to be not harmful or toxic to fish.
The recent study of Sipos (2015), investigating the acute toxicity of
zirconium dinitrate oxide in rainbow trout (Oncorhynchus mykiss)
according to OECD guideline 203, was considered as the key study for
endpoint coverage. In this study, a combined range finder/limit test was
performed with 1, 10 and 100% saturated solutions. The 100% saturated
solution was prepared using a nominal loading rate of 100 mg/L
(anhydrous zirconium dinitrate oxide). The pH of the initial solution
was adjusted to 6.0 in order to try to maximise quantities of dissolved
zirconium, while still remaining a physiologically acceptable pH level
for rainbow trout. After intense stirring for 24 h, pH was adjusted
again to 6.0 and the solution was filtered. The filtrate was then
further diluted to give 1 and 10% v/v dilutions of the saturated
solution. Dissolved zirconium concentrations above the LOQ of 0.03 mg
Zr/L could not be detected in any of the solutions throughout the test.
In addition, no mortality of fish was observed after 96 h in any of the
test solutions. Based on the results of this study, the 96-h LL50 was
concluded to be > 100 mg/L (anhydrous zirconium dinitrate oxide).
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