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EC number: 258-027-0 | CAS number: 52587-68-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Code number: FAT 21095/C
EN/Batch: 34
Purity: ca. 80 %
Appearance: black solid
Expiry date: 07/99 - Analytical monitoring:
- yes
- Details on sampling:
- Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at -18 °C to -22 °C until analysis.
- Vehicle:
- no
- Details on test solutions:
- Stock solution: 100.6 mg test substance were mixed and made up to 1000 ml with water
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test system: Daphnia magna
Strain: Straus 1820
Source: CIBA-GEIGY Ltd, testing facility
Number of daphnids: 20 per concentration and control. 4 replicates of 5 daphnia each.
Feeding: None during the test.
Breeding: Cultures of daphnia are maintained in glass vessels containing approx. 2.5L of reconstituted water at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN extract in such quantities that the food is consumed after 24h.
Pretreatment: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 µm sieve. This operation is repeated immediately before the exposure and the young (0- 24h of age) are retained for the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 240 mg CaCO3/l
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.9 - 8.0
- Nominal and measured concentrations:
- Nominal concentrations: 0.91, 2.0, 4.4, 9.6, 21 and 46 mg/l
Measured concentrations: 0.9, 2.0, 4.5, 10.1, 23.4, 53.3 mg/l - Details on test conditions:
- - Vessels: Beakers covered with watch glasses 100 ml solution per beaker.
- Water: Reconstituted water containing
- 65 mg NaHC03
- 294 mg CaCl2 • 2 H20
- 123 mg MgS04 • 7 H20
- 6 mg KCl
in 1000 ml bidistilled water.
The water was aerated with clean air for at least 24 hours before use.
- Aeration: None
- Lighting: Fluorescent light, 16 hours daily approx. 1500 lux
- Number of Daphnia: 20 per concentration and control. 4 replicates of 5 daphnids each. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 12.5 - 19.8 mg/L and Goodness of fit CHI is 4.59
- Details on results:
- Values observed
- NOEC (48 h): 2 mg/l
- EC 0 (48 h): 2 mg/l
- EC 100 (48 h): 46 mg/l
Controls
Immobilizations in blank: 0
Remarks:
The measured test substance concentrations were within the range of 100 ± 20 % of the nominal test concentrations, therefore values are based on nominal concentrations. - Reported statistics and error estimates:
- The LC-50 values were calculated according to the maximun likelihood method, probit model (Mc Cullagh, P., Neider, J.A., 1983: Generalized
linear models, Chapman & Hall, Lon. EC-values were graphically determined on gausso-logarithmic probability paper. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h EC50 of FAT 21095/C was determined to 15.6 mg/L.
- Executive summary:
The acute toxicity test on Daphnia magna (Straus 1820) was performed on test item FAT 21095/C according to the 92/69/EEC C.2 Acute toxicity for Daphnia method. 20 daphnids (0-24 hours old) were exposed to nominal concentrations of 0.91,2.0,4.4, 9.6, 21 and 46 mg test substance/L respectively. A static system was used for this test. Animals were observed for immobilization after 24 and 48 h. The measured test substance concentrations were within the range of 100 ± 20% of the nominal test concentrations, therefore values are based on nominal concentrations. Following results were obtained:
EC 50 ( 48 h) calculated: 15.6 mg/l
95 % confidence limit: 12.5 -19.8 mg/l
Goodness of fit CHI: 4.59
EC 50 ( 24 h) calculated: 57 mg/l
95 % confidence limit: 35.1-194 mg/l
Goodness of fit CHI: 3.34
NOEC ( 48 h ): 2.0 mg/l
EC 0 ( 48 h ): 2.0 mg/l
EC 100 (48h): 46 mg/l
Reference
Immobilization
(Initial numbers of daphnia = 20/test cone.)
Conc. Nominal | Number of Daphnia immobalized after 24 hrs exposure | |||||
mg/l | Vessel | Total | % | |||
1 | 2 | 3 | 4 | |||
Blank | 0 | 0 | 0 | 0 | 0 | 0 |
0.91 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | 0 | 0 |
4.4 | 0 | 0 | 0 | 1 | 1 | 5 |
9.6 | 0 | 0 | 0 | 0 | 0 | 0 |
21 | 3 | 1 | 0 | 0 | 4 | 20 |
46 | 1 | 2 | 3 | 3 | 9 | 45 |
Conc. Nominal | Number of Daphnia immobalized after 48 hrs exposure | |||||
mg/l | Vessel | Total | % | |||
1 | 2 | 3 | 4 | |||
Blank | 0 | 0 | 0 | 0 | 0 | 0 |
0.91 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | 0 | 0 |
4.4 | 0 | 0 | 0 | 1 | 1 | 5 |
9.6 | 1 | 0 | 0 | 1 | 2 | 10 |
21 | 3 | 5 | 2 | 4 | 14 | 70 |
46 | 5 | 5 | 5 | 5 | 20 | 100 |
Description of key information
The acute toxicity test on Daphnia magna (Straus 1820) was performed on test item FAT 21095/C according to the 92/69/EEC C.2 Acute toxicity for Daphnia method. 20 daphnids (0-24 hours old) were exposed to nominal concentrations of 0.91,2.0,4.4, 9.6, 21 and 46 mg test substance/L respectively. A static system was used for this test. Animals were observed for immobilization after 24 and 48 h. The measured test substance concentrations were within the range of 100 ± 20 % of the nominal test concentrations, therefore values are based on nominal concentrations. Following results were obtained:
EC 50 ( 48 h) calculated: 15.6 mg/l
95 % confidence limit: 12.5 -19.8 mg/l
Goodness of fit CHI: 4.59
EC 50 ( 24 h) calculated: 57 mg/l
95 % confidence limit: 35.1-194 mg/l
Goodness of fit CHI: 3.34
NOEC ( 48 h ): 2.0 mg/l
EC 0 ( 48 h ): 2.0 mg/l
EC 100 (48h): 46 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 15.6 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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