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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, non guideline study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
publication
Title:
Comparison of the Toxicity Thresholds of Water Pollutants to Bacteria, Algae, and Protozoa in Cell Multiplication Inhibition Test
Author:
Bringmann, G. and Kuhn, R.
Year:
1980
Bibliographic source:
Water Research 14(3) 231-241

Materials and methods

Principles of method if other than guideline:
The effect of the test substance on the inhibition of the multiplication of bacterial cells of the genus Pseudomonas was investigated. Cultures of the test organisms were exposed to a series of test concentrations of the test substance for 16 hours and the effects on cell multiplication measured using a turbidimetric technique. Results are reported relative to the blank controls.
GLP compliance:
no
Remarks:
predates GLP

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetic acid
EC Number:
200-580-7
EC Name:
Acetic acid
Cas Number:
64-19-7
Molecular formula:
C2H4O2
IUPAC Name:
acetic acid
Details on test material:
No data reported
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
The concentration of the bacterial suspension was measured turbidimetrically at 0 and 16 hours

Test solutions

Vehicle:
no
Details on test solutions:
Test solutions prepared from a stock solution, with each dilution containing 1 part v/v test substance solution in 2^0 to 2^14 v/v double distilled water. Nutrient medium, trace element solution and vitamin solution were added to each test vessel together with 10ml of bacterial suspension.

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Stock cultures of the test strain kept on the nutrient for stock and preliminary cultures in agar slant tubes. New stock cultures prepared at 1 week intervals. The inoculated stock culture incubated at 25°C for 24h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Post exposure observation period:
None

Test conditions

Hardness:
No data reported
Test temperature:
25°C
pH:
authors state that the solution of test substance was neutralised by the addition of a minimal volume of acid or alkaline solution
Dissolved oxygen:
No data reported
Salinity:
Not applicable
Nominal and measured concentrations:
No data reported
Details on test conditions:
Four parallel dilution series in 300ml Erlenmeyer flasks, stoppered with cotton-lined plastic caps. Both inoculated and non-inoculated dilution series left at 25°C for 16h
Reference substance (positive control):
no
Remarks:
No data reported

Results and discussion

Effect concentrations
Duration:
16 h
Dose descriptor:
NOEC
Effect conc.:
1 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
After 16 hours the extinction of the monochromatic radiation at 436nm in a 10mm layer in the inoculated dilution series was measured. The lowest concentration having a mean extinction value ≥3 below the mean for non-toxic dilutions is reported as the EC3.
Results with reference substance (positive control):
No data reported
Reported statistics and error estimates:
No data reported

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Remarks:
non-standard test
Conclusions:
An 16h EC3 of 850mg/l has been reported for Pseudomonas putida . This can be considered to be the NOEC.
Executive summary:

An 16h EC3 of 850mg/l has been reported for Pseudomonas putida . This can be considered to be the NOEC. Although this study is non-GLP and is non guideline, it is considered suitable for use as a key study. The study investigates the effects of the test substance on a standard test organism. A series of exposure concentrations were used and results reported as the inhibition of culture growth relative to controls.