Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
fish, juvenile growth test
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: The study report has not been received and therefore cannot be reviewed and evaluated in full.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetic acid
EC Number:
200-580-7
EC Name:
Acetic acid
Cas Number:
64-19-7
Molecular formula:
C2H4O2
IUPAC Name:
acetic acid
Details on test material:
Acetic acid technical (60%). Lot number = A07ET265
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not appliacble

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Analysis was done for 1 and 24h after renewal and after 21 days.
Effects monitored daily.
Average oxygen content, pH value and temperature were checked at the beginning, after 2, 5, 7, 12, 14, 16, 19 and 21 days and at the end of the test.
Sampling of the test solution of each test concentrations for analysis and waste disposal was performed at 0, 24, 48, 72 and 96 hours after application.

Test solutions

Vehicle:
no
Details on test solutions:
Eight renewals of the test solutions during the test period.
test solution diluted with water.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
size: 3.5-5.4cm

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
Not applicable

Test conditions

Hardness:
Not reported
Test temperature:
15.8-18-8°C
pH:
4.49-8.03
Dissolved oxygen:
8.3-9.0 mg/l
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 0, 13.2, 19.8, 29.6, 44.4, 66.7, 100.0, 150.0, 225.0. Concentrations were measured. No toxicity effect in the concentrations in 13.2, 19.8, 29.6 and 44.4mg/l, these samples have not been analysed.
Details on test conditions:
photoperiod: alternating 16 hour light and 8 hours dark per day

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
57.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
87 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
52.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: For 100% acetic acid
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
34.3 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: For 100% acetic acid
Details on results:
LC50 values were derived from aluclations considering the geometrical means of LC0 and LC100
Results with reference substance (positive control):
No data reported

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
criteria met
Conclusions:
The mean measured 21d LC50 and NOEC for 60% acetic acid was, respectively, 87mg/l and 57.2mg/l. The mean measured 21d LC50 and NOEC for 100% acetic acid was, respectively, 52.2mg/l and 34.3mg/l.
Executive summary:

The mean measured 21d LC50 and NOEC for 60% acetic acid was, respectively, 87mg/l and 57.2mg/l. The mean measured 21d LC50 and NOEC for 100% acetic acid was, respectively, 52.2mg/l and 34.3mg/l. This study is GLP compliant and follows a standard guideline. It is considered reliable and suitable for use as a key study.