Butan-1-ol

Specific investigations: other studies

Administrative data

Endpoint:

behavioural effects

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Concentration-dependent behavioral changes in mice following short-term inhalation exposure to various industrial solvents

Author:

De Ceaurriz J.C. et al.

Year:

1983

Bibliographic source:

Toxicol. appl. Pharmacol. 67, 383- 389 (1983); citred in Environmental Health Criteria 65, Butanols (1987)

Materials and methods

Principles of method if other than guideline:

Male mice were tested in a "behavioural despair" swimming test after inhalative exposure.

GLP compliance:

not specified

Type of method:

in vivo

Endpoint addressed:

neurotoxicity

Test material

Test material information

Constituent 1

Reference substance name:

Butan-1-ol

EC Number:

200-751-6

EC Name:

Butan-1-ol

Cas Number:

71-36-3

Molecular formula:

C4H10O

IUPAC Name:

butan-1-ol

Test material form:

liquid

Test animals

Species:

mouse

Strain:

Swiss

Remarks:

OF1 mice

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: IFFA CREDO, Domaine des Oncins, Saint-Germain-sur-I'Arbresle, France
- Weight at study initiation: 20-25g
- Diet (e.g. ad libitum): food (UAR-Alimentation,Villemoisson, France) ad libitum
- Water (e.g. ad libitum):ad libitum
- Acclimation period: 7days

Administration / exposure

Route of administration:

inhalation: vapour

Details on exposure:

Exposures were conducted in 200-liter (stainless steel) inhalation chambers designed to sustain dynamic and adjustable
flows of air (10 to 30 m' per hour) . To prevent leakage of the test atmospheres into the room, the chambers
were maintained at a negative Pressure of 1 to 2 mm H2O,relative to the room.The vapors of the test compounds
were injected at the entry of the inhalation chambers.
For generation of organic compound-air mixtures, two systems were utilized ; an injector was employed and heat was added as needed to obtain the vaporization of the substances, or vapors were produced by bubbling an additional air flow through a vial containing the liquid compound . Under both conditions, the vaporized compound was carried into a mixing device for further diIution with air to the final concentration before entering the exposure chamber.

Duration of treatment / exposure:

4 h

Frequency of treatment:

single

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

470 ppm

Remarks:

1446.7 mg/m3

Doses / concentrations 2

Dose / conc.:

548 ppm

Remarks:

1686.7mg/m3

Doses / concentrations 3

Dose / conc.:

844 ppm

Remarks:

2597.8 mg/m3

Doses / concentrations 4

Dose / conc.:

965 ppm

Remarks:

2970.2 mg/m3

No. of animals per sex per dose:

10 males/ treatment

Control animals:

yes

Details on study design:

At the end of each exposure period:
- mouse immersed inside a vertical glass cylinder placed in a macrolon cage containing 10 cm of water at 21 to 23°C
- total duration of immobility observed during the first 3 min after plunging the mouse in water measured
- mouse was judged to be immobile when it ceased struggling and remained floating passively in the water in a semihorizontai position only making movements necessary to keep its head above the water.

Results and discussion

Details on results:

Compared with controls, a dose-related decrease in the duration of immobility measured over a 3-min period was observed (see table).The mean active atmospheric concentration associated with a 50% decrease in immobility (ID50) for n-butanol was determined to be 617 ppm [95% confidence interval 547-681].

Any other information on results incl. tables

exposure concentration (ppm)	control groups (duration of immobility)	exposed groups (duration of immobility)	Percentage change
470	91.9±6.3	56.6± 8.0 *	-38
548	100.9± 5.3	53.2± 2.5 *	-47
844	92.8± 7.7	37.1± 10.5*	-60
965	79.2± 5.9	24.0± 4.9 *	-70

The values given are the mean durations  ±SE obtained from 10 animals and expressed in sec.

*Significantly different from the respective control values, p < 0 .05