2,2',2''-nitrilotriethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: Evaluation of 108 chemicals

Author:

Galloway S.M. et al.

Year:

1987

Bibliographic source:

Environ.Molec.Mutag. 10 Suppl.10,|1-175 (1987)

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

2004

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)

Principles of method if other than guideline:

Method: other: after Galloway, S.M. et al.: Environ. Mutagen. 7, 1-51

GLP compliance:

not specified

Type of assay:

in vitro mammalian chromosome aberration test

Test material

Test material information

Constituent 1

Reference substance name:

2,2',2''-nitrilotriethanol

EC Number:

203-049-8

EC Name:

2,2',2''-nitrilotriethanol

Cas Number:

102-71-6

Molecular formula:

C6H15NO3

IUPAC Name:

2-[bis(2-hydroxyethyl)amino]ethan-1-ol

Method

Species / strain

Species / strain / cell type:

Chinese hamster Ovary (CHO)

Details on mammalian cell type (if applicable):

Cloned Chinese hamster ovary cells (CHO-W-B1) were cultured in Mc-Coy's 5a medium with 10% fetal calf serum, L-glutamine, and antibiotics.

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254 induced rat liver

Test concentrations with justification for top dose:

1510 - 4030 µg/mL (without S9)
6040 - 10100 µg/mL (with S9)
Doses were chosen for the aberration test based on a preliminary test of cell survival 24 hr after treatment.

Vehicle / solvent:

Water, dimethyl sulfoxide (DMSO), ethanol, or acetone, in that order of preference.

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: triethylenemielamine, mitomycin C. or cyclophosphamide

Results and discussion

Test results

Key result

Species / strain:

Chinese hamster Ovary (CHO)

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified