Trichloro(methyl)silane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to a test protocol that is comparable to the appropriate OECD test guideline. It was not compliant with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

not specified

Remarks:

Although a GLP statement was not included with the report, it is known that Bushy Run Research Center (BRRC) operated as a GLP-facility at the time the testing was conducted.

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trichloro(methyl)silane

EC Number:

200-902-6

EC Name:

Trichloro(methyl)silane

Cas Number:

75-79-6

Molecular formula:

CH3Cl3Si

IUPAC Name:

trichloro(methyl)silane

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS

- Weight at study initiation: 200-300g

- Fasting period before study: overnight

- Diet: commercial diet, ad libitum

- Water: municipal water, ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Silicone L-45 oil

Details on oral exposure:

Hiltop-Wistar albino rats received the test material as a 25% dilution in Silicone L-45 oil by stomach intubation with a ball-end stainless steel needle.

Doses:

The test material was administered undiluted t first at 0.25 ml/kg to males and 1 ml/kg to females. It was then administered as a dilution in Silicone L-45 Oil as follows; 8, 4, 2, 1, and 0.5 mL/kg in males; 8, 4, 2, and 1 mL/kg in females.

No. of animals per sex per dose:

5 animals/sex/dose for a total of 45 animals

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

- Frequency of observations and weighing: Animal weights were recorded at 0 days (before dose), 7 days and 14 days (just prior to sacrifice).

- Necropsy of survivors performed: yes

- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Statistics:

LD50s were calculated by the moving average method (Thompson, 1947) and were based on a 14-day observation period.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

1.62 mL/kg bw

95% CL:

0.903 - 2.92

Remarks on result:

other: sample dosed as a 25% dilution in Silicone L-45 oil

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

2.83 mL/kg bw

95% CL:

2.09

Remarks on result:

other: sample dosed as a 25% dilution in Silicone L-45 oil

Effect levels 3

Sex:

male

Dose descriptor:

LD50

Effect level:

< 0.25 mL/kg bw

Remarks on result:

other: Sample dosed undiluted

Effect levels 4

Sex:

female

Dose descriptor:

LD50

Effect level:

< 1 mL/kg bw

Remarks on result:

other: Sample dosed undiluted

Mortality:

See Table 1 and 2.

Clinical signs:

other: MALES Dosage: 8 mL/kg: Dyspnea at 3 min; death at 7 to 20 min Dosage: 4 mL/kg: sluggishness, dyspnea at 30 min Dosage: 2 mL/kg: Death of 2 at 4 hr Dosage: 1 mL/kg: None noted before death Dosage: 0.5 mL/kg: None noted FEMALES Dosage: 8 mL/kg: Sluggishnes

Gross pathology:

MALES
Dosage: 8 mL/kg: Stomach distended, gas and liquid-filled, injected, walls brittle, glandular portions black; livers brown, firm; pancreas brown; intestines brown and firm near stomach, lower areas red; kidneys brown, firm; spleens enlarged
Dosage: 4 mL/kg: In victims, lungs red; stomach of 1 perforated; livers pale, firm; intestines injected; adrenals red. In survivors, nothing remarkable
Dosage: 2 mL/kg: In victims, clear fluid in thoracic cavity; stomachs enlarged, black, 1 perforated; intestines brown; fluid in abdominal cavity; spleens enlarged. In survivor, nothing remarkable
Dosage: 1 mL/kg: In victims, lungs pink or red. In survivors, nothing remarkable
Dosage: 0.5 mL/kg: Nothing remarkable

FEMALES
Dosage: 8 mL/kg: Stomachs liquid-filled, injected, glandular sections black, firm; livers tan, firm near stomach; intestines tan and firm near stomach, red and injected in lower areas; kidneys tan, firm
Dosage: 4 mL/kg: Similar to above; 1 stomach perforated; 2 with fluid in abdominal cavity
Dosage: 2 mL/kg: Lungs with dark red patches
Dosage: 1 mL/kg: Nothing remarkable

Any other information on results incl. tables

Table 1: Number of animals dead and time range within which mortality occurred when dosed with undiluted sample. 

Dose (ml/kg bw)	Mortality (# dead/total)			Time range of deaths (day)
	Male	Female	Combined
0.25	5/5	n/a	5/5	0,1,2
1.0	n/a	4/5	4/5	0

 

Table 2: Number of animals dead and time range within which mortality occurred when dosed with diluted sample

Dose (ml/kg bw)	Mortality (# dead/total)			Time range of deaths (hours)
	Male	Female	Combined
0.5	0/5	n/a	0/5	-
1.0	2/5	0/5	2/10	1,2
2.0	4/5	0/5	4/10	0,1,2
4.0	3/5	5/5	8/10	1,2,5
8.0	5/5	5/5	10/10	0

 

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 values of <320 mg/kg for males and <1280 mg/kg for females were determined in a reliable study conducted according to an appropriate test protocol. Not conducted according to GLP.