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EC number: 270-112-4 | CAS number: 68411-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24. Feb. 2010 - 13. Apr. 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant Breisgauer Bucht was used as inoculum with a concentration corresponding to 30 mg dry solids per litre. The treatment plant clarifies predominantly domestic wastewater and has a capacity of 600.000 inhabitant equivalents. Sampling date of activated sludge was on march 2nd, 2010. Dry solid of the activated sludge was 5.72 g/l by weight measurements after 2 h drying at 105°C (mean of triplicate measurements). The activated sludge was washed twice by settling the sludge, decanting the supernatant and re-suspending the sludge in tap water.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 15.67 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to relevant OECD TG
- Test temperature: 20 - 21.5 °C
- pH adjusted: no
- Aeration of dilution water: aerated with CO2-free air at a rate of 30-100 ml/min overnight
- Suspended solids concentration: 30 mg dry solids/L
TEST SYSTEM
- Culturing apparatus: glas containers (2000 mL gas wash bottles)
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: aeration
- Measuring equipment: total carbon analyser (TOC-5000A Shimadzu with an autosampler ASI-5000A)
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: two CO2-absorber flasks connected in line with 0.2 M NaOH
- Other:
SAMPLING
- Sampling frequency: 8 samling dates
- Sampling method: 4 ml NaOH from the first of two CO2-absorber flasks connected in line was sampled and the IC's were determined
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3
- Reference substance: 3
- Other: - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 102.8
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 day window criterion satisfied
- Details on results:
- The degradation extent of the test item was 102.8% within 28 days after acidification (mean of three replicates). For finding the exact position of the 10-d-window the degradation extents of the days without measurement were calculated by interpolation.
On day 1 the calculated mean degradation extent of the test item was for the first time higher than 10% (mean value: 12.1%). According, the end of the 10-d window is on day 11. On day 7 the mean degradation extent of the test item already reached 81.9%. Therefore the test item reached the pass level for ready biodegradability (60% ThCO2 and 10 d-window).
The highest mean CO2-evolution of the blank flasks was 29.6 mg/l within 28 days after acidification. - Results with reference substance:
- The reference compound sodium benzoate reached the pass levels for ready
biodegradability within 4 days - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The degradation of the test item was 102.8% within 28 days (after acidification). The test item reached the criterion for ready biodegradability.
- Executive summary:
Benzoic acid, C12 -15 -alkyl esters was tested in a Modified Sturm Test according to the OECD Guideline for the Testing of Chemicals, No.: 301 B, July 17th, 1992: Ready Biodegradability, CO2Evolution Test (Modified SturmTest) for 28 days (and 29 days after acidification) to determine whether the test substance is readily biodegradable. In the Modified Sturm Test the evolution of carbon dioxide shows the degree of mineralisation of the test substance. Degradation was followed over 28 days by determining the carbon dioxide (CO2) produced. The CO2was trapped in sodium hydroxide solution which was measured for its inorganic carbon content on 8 sampling dates. The amount of carbon dioxide produced from the test substance (corrected for that derived from the blank inoculum) was expressed as a percentage of the theoretical carbon dioxide production (ThCO2).The test was carried out with one concentrations of the test substance corresponding to 20 mg carbon per litre. At the end of the exposure period a degradation rate of 102.8 % of the Theoretical CO2(ThCO2) of the test substance was measured. The pass level (= 60 % ThOD for respirometric methods according to the OECD-Guideline 301) was reached within the 10-day time window. The test substance can be considered to be readily biodegradable within a test period of 28 days.
Reference
Ultimate Biodegradation after x days (% of ThCO2)
Reactor |
Day |
0 |
4 |
7 |
11 |
14 |
21 |
28 |
29 |
|
|
|
|
|
|
|
|
|
|
7 |
Test flasks |
0 |
58.6 |
88.6 |
101.8 |
104.7 |
105.3 |
108.0 |
108.2 |
8 |
0 |
43.1 |
79.7 |
92.3 |
95.1 |
96.9 |
100.2 |
100.9 |
|
9 |
0 |
45.3 |
77.4 |
89.6 |
92.6 |
95.0 |
99.6 |
99.3 |
|
4 |
Reference flasks |
0 |
82.1 |
93.7 |
100.5 |
101.2 |
101.1 |
105.1 |
103.2 |
5 |
0 |
81.1 |
92.9 |
95.8 |
99.8 |
101.2 |
102.0 |
100.6 |
|
6 |
0 |
81.2 |
94.5 |
98.9 |
102.0 |
102.2 |
102.6 |
104.5 |
Description of key information
The degradation of the test item was 102.8% within 28 days (after acidification). The test item reached the criterion for ready biodegradability.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Benzoic acid, C12 -15 -alkyl esters was tested in a Modified Sturm Test according to the OECD Guideline for the Testing of Chemicals, No.: 301 B, July 17th, 1992: Ready Biodegradability, CO2Evolution Test (Modified SturmTest) for 28 days (and 29 days after acidification) to determine whether the test substance is readily biodegradable. In the Modified Sturm Test the evolution of carbon dioxide shows the degree of mineralisation of the test substance.Degradation was followed over 28 days by determining the carbon dioxide (CO2) produced. The CO2was trapped in sodium hydroxide solution which was measured for its inorganic carbon content on 8 sampling dates. The amount of carbon dioxide produced from the test substance (corrected for that derived from the blank inoculum) was expressed as a percentage of the theoretical carbon dioxide production (ThCO2).The test was carried out with one concentrations of the test substance corresponding to 20 mg carbon per litre. At the end of the exposure period a degradation rate of 102.8 % of the Theoretical CO2(ThCO2) of the test substance was measured. The pass level (= 60 % ThOD for respirometric methods according to the OECD-Guideline 301) was reached within the 10-day time window. The test substance can be considered to be readily biodegradable within a test period of 28 days. |
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