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EC number: 204-634-0 | CAS number: 123-54-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-04-03 to 1985-12-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Conduction and documentation of study very acceptable. Study report available.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Principles of method if other than guideline:
- Test performed according to standard protocols by inclusion of a metabolic activation system (S9-Mix from livers of Sprague-Dawley rats pretreated with Aroclor 1254).
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Pentane-2,4-dione
- EC Number:
- 204-634-0
- EC Name:
- Pentane-2,4-dione
- Cas Number:
- 123-54-6
- Molecular formula:
- C5H8O2
- IUPAC Name:
- pentane-2,4-dione
- Details on test material:
- - Name of test material (as cited in study report): 2,4-Pentanedione
- Physical state: liquid
- Expiration date of the lot/batch: not reported
- Stability under test conditions: stable
Constituent 1
Method
- Target gene:
- not applicable
Species / strain
- Species / strain / cell type:
- other: S. typhimurium, TA 98, 100, 1535, 1537, 1538
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with
- Metabolic activation system:
- S9-Mix
- Test concentrations with justification for top dose:
- 0.3 - 30mg/plate
- Vehicle / solvent:
- water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4 -nitro-o-phenylenediamine
- Remarks:
- TA98 and TA1538 without S9
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA100 without S9
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA1537 without S9
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- all strains with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:in agar (plate incorporation)
DURATION
- Exposure duration: 24 and 48 h
NUMBER OF REPLICATIONS:3
DETERMINATION OF CYTOTOXICITY
- Method: growth inhibition
- Evaluation criteria:
- Test chemicals which produce at least a 2-fold and dose-related increase in mutant colonies over the concurrent solvent control value are considered to be bacterial mutagens and suspect mammalian mutagens.
- Statistics:
- not reported
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium, TA 98, 100, 1535, 1537, 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 97.8 mg/plate produced significant cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: none reported
- Effects of osmolality: none reported
- Evaporation from medium: none reported
- Water solubility: soluble
- Precipitation: none reported
- Other confounding effects: none reported
RANGE-FINDING/SCREENING STUDIES: yes, outside GLP
COMPARISON WITH HISTORICAL CONTROL DATA: yes, compliant - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: S. typhimurium, TA 98, 100, 1535, 1537, 1538
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
From the results it is concluded that 2,4-pentanedione is not mutagenic under the conditions of the assay. - Executive summary:
2,4-pentanedione (99.2% pure) was tested for potential mutagenic activity using the Salmonella/microsome bacterial mutagenicity assay (Ames test).
The test was performed according to standard protocols by inclusion of a metabolic activation system (S9-Mix from livers of Sprague-Dawley rats pretreated with Aroclor 1254). In preliminary trials with strain TA 100 only, a concentration of 97.4 mg/plate proved to completely inhibit bacterial growth. The next lower concentration of 30 mg/plate showed some toxic effects. Accordingly, doses selected on the basis of these trials were 0.3, 1.0, 3.0, 10.0 and 30.0 mg/plate. Incubations were run in triplicate at 37°C for 24 to 48 hours. Plates were examined for the condition of their background lawns and growth was recorded as either confluent (non-toxic), sparse (moderately toxic) or absent (toxic). The solvents of choice was water. Concurrent solvent and positive controls were run in each test. Positive control substances were without S9-mix 0.01 mg 4 -nitro-ophenylenediamine/ plate for TA98 and TA1538, 0.01 mg sodium azide for TA100, 0.06 mg 9-aminoacrididine/plate for TA1537 and with S9-mix 0.01 mg 2-aminoanthracene for all strains.2,4-pentanedione did not produce a doubling or a dose-response relationship of the number of revertants/plate in the Salmonella typhimurium strains used neither in the absence nor in the presence of a metabolic activation system. Dose selection appeared to be in a suitable range, because in tests both with and without metabolic activation, bacteriotoxicty was observe d at 30 mg/plate (highest dose tested) with all strains. Well proven positive controls did produce mutagenic effects demonstrating the functionality of the test system. It can therefore be concluded that 2,4-pentanedione is not mutagenic under the conditions of the assay.
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