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EC number: 232-077-3
CAS number: 7785-26-4
- Concentrations measured in freshly
prepared solutions and in 24-h old solutions revealed that the
concentration of the test item was not satisfactorily maintained within
± 20 % of the initial concentration throughout the test (except some
concentrations the second day). Since the deviation of the exposure
concentrations of the test substance was greater than ± 20 % of the
initial concentrations, the results were expressed in terms of geometric
means of the exposure concentrations.
- See table 6.1.1/1 for more details.
6.1.1/1. Mean measured test item concentrations (mg/L) in test water -
0 - 24 h
(Day 0 – Day 1)
24 - 48 h
(Day 1 – Day 2)
48 - 72 h
(Day 2 – Day 3)
72 - 96 h
(Day 3 – Day 4)
0 - 96 h
(Day 0 – Day 4)
In an acute aquatic toxicity study performed
according to OECD guideline 203 and in compliance with GLP, groups
(7/concentrations) of zebrafish (Danio rerio) were exposed to
(-)-alpha-pinene at concentrations of 0 (blank), 0.08, 0.14, 0.19, 0.26
and 0.58 mg/L [measured (geometric mean)] for 96 h under semi-static
conditions. Mortalities were recorded at 3, 6, 24, 48, 72 and 96 h. A
preliminary range-finding test was conducted with 3 fishes/concentration
at 0.5, 1.0 and ca. 2.0 mg/L (nominal) and cumulative mortalities after
96 h were recorded as 66.7, 100 and 100 %, respectively.
In the main test, mortalities (96 h) at 0
(blank), 0.08, 0.14, 0.19, 0.26 and 0.58 mg/L mg/L (measured) were 0, 0,
0, 0, 14.3 and 100 %, respectively. LC50 values at 24, 48 and 72-96 h
were calculated to be 0.632, 0.392 (0.291-0.591) and 0.303 (0.187-0.578)
Therefore, the 96h-LC50 for (-)-alpha-pinene
was 0.303 (0.187-0.578) mg/L in zebrafish (Danio rerio).
96h-LC50 for freshwater fish for (-)-alpha-pinene is 0.303 mg/L.
One reliable study is available for the
substance itself and is selected as key study. Fish (Danio rerio) were
exposed to (-)-alpha-pinene at nominal test concentrations of 0.10,
0.20, 0.30, 0.60 and 1.00 mg/L over a test period of 96 hours
(semi-static test, daily renewal, closed conditions). Concentrations
were analytically verified at the start and daily until the end of the
test. The concentration of the test item was not satisfactorily
maintained within ± 20% of the initial concentration throughout the
test. Thus, the endpoint were based on the geometric means of the
measured concentrations. The 96h-LC50 was determined to be 0.303 mg/L,
the LC0 and LC100 were 0.187 mg/L and 0.578 mg/L, respectively.
the 96h-LC50 of 0.27 mg/L is valid for key value for chemical safety
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