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EC number: 287-335-8
CAS number: 85480-55-3
Table 1: Mean OD570 values and
viabilities for the negative control, positive control and test
substance (Study# 98499)
OD = Optical density
* = The mean percentage viability of the
negative control tissue is set at 100%
na = Not applicable
An in vitro study was conducted to
evaluate the corrosivity potential of benzenesulfonic acid, mono-
C10-13-alkyl derivs., MEA salts (CAS RN 85480-55-3; 19.01% active)
according to OECD guideline 431 and EU Method B.40bis using the EpiDerm™
Human Skin Model.
Duplicate EpiDerm™ tissues were treated with
the test substance for exposure periods of 3 and 60 minutes. Negative
control (sterile distilled water) and positive control (8.0 N potassium
hydroxide) groups were also treated for each exposure period. At the end
of the exposure period, the tissues were rinsed, before each tissue was
taken for MTT-loading. After MTT loading each tissue was placed in 2 mL
isopropanol for MTT extraction.
At the end of the formazan extraction period
each well was mixed thoroughly and triplicate 200 μL samples were
transferred to the appropriate wells of a pre-labelled 96-well plate.
The optical density (OD) was measured at 570 nm (OD570).
The acceptance criteria for negative control
and positive control groups were met in the study. The mean OD570 for
negative control treated tissues was 1.737 for the 3-minute exposure
period and 1.815 for the 60-minute exposure period. The mean viability
for the positive control treated tissues were 5.3 and 3.7% for the 3 and
60 minute exposure periods, respectively. The coefficient of variation
(CV) between tissue replicates did not exceed 30%.
The relative mean viabilities for test
substance were 107.5 and 62.6% for 3 and 60 minutes exposure periods,
respectively. Based on the mean viabilities, the test substance was
categorized as non-corrosive.
In an in vitro test for skin
corrosion using the EpiDerm™ Human Skin Model, benzenesulfonic acid,
mono- C10-13-alkyl derivs., MEA salts (CAS RN 85480-55-3; 19.01% active)
was considered to be non-corrosive to the skin after treatment periods
of 3 and 60 minutes.
This skin corrosion test is classified as
acceptable and satisfies the guideline requirement for the current OECD
guideline 431 (In vitro skin corrosion: Reconstructed Human
Epidermis (RhE) test method).
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