Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
calculation (if not (Q)SAR)
Remarks:
Migrated phrase: estimated by calculation
Adequacy of study:
key study
Study period:
2004
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Fisons
- Type: Pump: 600E (Waters), injector: 717plus (Waters)
- Type, material and dimension of analytical (guard) column: Hypersil CN (Interchim) particle size 5 μm, length 250mm, inner diameter 4.6 mm
- Detection system: UV; (2487 Waters, wavelength 210 nm


MOBILE PHASES
- Type: methanol - Milli-Q water
- Experiments with additives carried out on separate columns: no
- pH: 6.40
- Solutes for dissolving test and reference substances: acetamide, methylbenzoate, naphtalene, 1,2,3-trichlorobenzene, phenantrene, DTT


DETERMINATION OF DEAD TIME
- injection of a solution of formamide was performed in duplicate (2040 ng)


DETERMINATION OF RETENTION TIMES injected volume: 20 μl
- Quantity of test substance introduced in the column: 104 ng
- Quantity of reference substances: acetamide 74 ng
methylbenzoate 108 ng
naphtalene 15 ng
1,2,3-trichlorobenzene 76 ng
phenantrene 36 ng
DTT 400 ng
- Intervals of calibration: before and after injections


REPETITIONS
- Number of determinations: 2


EVALUATION
- Calculation of capacity factors k': k' = (tr-t0) / t0 (t0: HPLC dead time, tr: HPLC retention timeof the items)
- Determination of the log Koc value: the log k' data of the reference items were plotted against their log Koc values
from batch equilibrum. using this plot, the Koc values determined for the reference
items were within ± 0.5 log units of the values determined by the batch equilibrum
method in the guideline and the log k' value of the test item was then used to
determine its log Koc value. (within 0.25 log units)

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
>= 1 229 - <= 1 231
Type:
log Koc
Value:
>= 3.08 - <= 3.1

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: formamide 3.32
acetanilide 3.90
methylbenzoate 4.38
naphtalene 5.64
1,2,3-trichlorobenzene 6.35
phenantrene 8.82
DTT 16.86
- Average retention data for test substance: 5.89

Applicant's summary and conclusion